LOCUS Exported 5931 bp ds-DNA circular SYN 26-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5931
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 4..1670
/label=CAG promoter
regulatory 1758..1767
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1857..1880
/codon_start=1
/product="8xHis affinity tag"
/label=8xHis
/translation="HHHHHHHH"
misc_feature 1881..2981
/label=MBP
CDS 3015..3035
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
CDS 3066..3083
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 3111..3134
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 3153..3179
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
polyA_site 3353..3408
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
rep_origin complement(4181..4769)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4940..5800)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5801..5905)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attgcggccg tctcaggccg
1741 aattcgtcga caagcttgcc accatgggga tccttcccag ccctgggatg cctgcgctgc
1801 tctccctcgt gagccttctc tccgtgctgc tgatgggttg cgtagctgaa accggtcacc
1861 atcaccatca ccatcaccat aaaaccgaag aaggtaaact ggtaatctgg attaacggcg
1921 ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta
1981 aagtcaccgt tgagcatccc gataaactgg aagagaaatt cccacaggtt gcggcaactg
2041 gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac gctcaatctg
2101 gcctgttggc tgaaatcacc ccggccgcag cgttccagga caagctgtat ccgtttacct
2161 gggatgccgt gcgctacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttgt
2221 ccctgattta taacaaggat ctgctgccga acccgccaaa aacctgggaa gagatcccgg
2281 cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac
2341 cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag tatgcagccg
2401 gaaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct
2461 tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag
2521 aacatgcctt taatcatggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca
2581 acatcgacac cagcgcagtg aattatggtg taacggtgct gccgaccttc aagggtcaac
2641 catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt ccgaacaaag
2701 agctggcaaa agagttcctc gaaaactacc tgctgactga tgaaggtctg gaagcggtta
2761 ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag ttggtgaaag
2821 atccacgtgt tgccgccaca atggaaaacg cccagaaagg tgaaatcatg cccaacatcc
2881 cgcagatgtc cgctttctgg tatgccgtgc gcactgcggt gattaacgcc gccagcggtc
2941 gtcagactgt cgatgcagcc ctggcagccg cgcagactaa tatcaccaag ggcggaggga
3001 gcggcggagg gagcgaaaac ctgtattttc agagcgcggc cgccgcgagc tcggctagcc
3061 tcgagcacca tcaccatcac cattgataaa cgcgtttaat taaccccggg tggagccacc
3121 cccagttcga gaagtgataa gatgatcaag gatatcccta cgacgtgccc gactacgcct
3181 gataatctag aactagtatc gcgataattc actcctcagg tgcaggctgc ctatcagaag
3241 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
3301 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
3361 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
3421 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
3481 tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa
3541 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
3601 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
3661 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
3721 cttatgaaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc
3781 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
3841 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
3901 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatcga tccgctgcat taatgaatcg
3961 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
4021 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
4081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
4141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
4201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
4261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
4321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
4381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
4441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
4501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
4561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
4621 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
4681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
4741 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
4801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
4861 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
4921 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
4981 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
5041 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
5101 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
5161 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
5221 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
5281 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
5341 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
5401 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
5461 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
5521 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
5581 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
5641 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
5701 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
5761 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
5821 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
5881 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
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