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pBAD-Myc-HisA pBAD/Myc-HisA大肠pBAD系列表达质粒

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产物名称: pBAD-Myc-HisA pBAD/Myc-HisA大肠pBAD系列表达质粒
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简单介绍

pBAD-Myc-HisA pBAD/Myc-HisA大肠pBAD系列表达质粒是衍生于pBR322载体。载体设计用来在大肠杆菌中进行可调节，剂量依赖性的表达和纯化重组目的蛋白。pBAD-Myc-HisA pBAD/Myc-HisA大肠pBAD系列表达质粒使用大肠杆菌araBAD启动子（pBAD）增强了大肠杆菌重组蛋白可溶性表达的水平。pBAD/His和pBAD/Myc His载体上的调节蛋白AraC能够调控pBad启动子。


pBAD-Myc-HisA pBAD/Myc-HisA大肠pBAD系列表达质粒  的详细介绍
 

pBAD-Myc-HisA pBAD/Myc-HisA大肠pBAD系列表达质粒

  • 产物信息
  • 基本信息
  • 质粒介绍
  • 质粒图谱
  • 质粒序列

产物信息

产物货号 产物名称 产物规格 优惠价
XY0812 pBAD-Myc-HisA pBAD/Myc-HisA大肠pBAD系列表达质粒

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使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产物的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子: araBAD promoter
复制子: PBR322 ori
质粒分类: 大肠杆菌载体；辫叠础顿系列表达质粒
质粒大小: 4094bp
质粒标签: C-6x His ,C-Myc Epitope
原核抗性: 氨苄青霉素础尘辫
克隆菌株: 大肠杆菌顿贬5α
培养条件: 37℃，有氧，尝叠
诱导方式: 阿拉伯糖
5'测序引物: pBAD-F: ATGCCATAGCATTTTTATCC
3'测序引物: pBAD-R: GATTTAATCTGTATCAGG
备注: pBAD/Myc-His A 载体是阿拉伯糖调控载体；在无葡萄糖的培养基中，阿拉伯糖正向调控目的基因的表达；通过调节阿拉伯糖的浓度水平来优化目的蛋白的可溶性表达

质粒介绍

        pBAD/His和PBAD/Myc-His载体质粒是衍生于pBR322载体。载体设计用来在大肠杆菌中进行可调节，剂量依赖性的表达和纯化重组目的蛋白。使用大肠杆菌araBAD启动子（pBAD）增强了大肠杆菌重组蛋白可溶性表达的水平。pBAD/His和pBAD/Myc His载体上的调节蛋白AraC能够调控pBad启动子。

质粒图谱


质粒序列

LOCUS       Exported                4094 bp ds-DNA     circular SYN 11-SEP-2016
DEFINITION  synthetic circular DNA
KEYWORDS    pBAD Myc-HisA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4094)
  TITLE     Direct Submission
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..285
                     /gene="araBAD"
                     /note="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             377..406
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     CDS             422..439
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      665..751
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      843..870
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        889..980
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             981..1841
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2012..2600
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    2786..2926
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(3190..4068)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /note="araC"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat ggatccgagc tcgagatctg cagctggtac catatgggaa
      361 ttcgaagctt gggcccgaac aaaaactcat ctcagaagag gatctgaata gcgccgtcga
      421 ccatcatcat catcatcatt gagtttaaac ggtctccagc ttggctgttt tggcggatga
      481 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
      541 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
      601 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
      661 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      721 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
      781 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
      841 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
      901 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      961 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1021 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1081 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1141 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1201 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     1261 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1321 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1381 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1441 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1501 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1561 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1621 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1681 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1741 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1801 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1861 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1921 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1981 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2041 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2101 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2161 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2221 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2281 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2341 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2401 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2461 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2521 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2581 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2641 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2701 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2761 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     2821 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     2881 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     2941 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3001 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3061 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat
     3121 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt
     3181 cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
     3241 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
     3301 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
     3361 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
     3421 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
     3481 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
     3541 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
     3601 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
     3661 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
     3721 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
     3781 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
     3841 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
     3901 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
     3961 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
     4021 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
     4081 cccgccattc agag
//
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