phospho-ALK (Tyr1604)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 3022R
英文名称phospho-ALK (Tyr1604)
中文名称磷酸化间变型**瘤激酶抗体
别 名ALK (Phospho-Tyr1604); ALK(phospho-Y1604); p-ALK(Tyr1604); p-ALK(Y1604); ALK tyrosine kinase receptor precursor; ALK/EML4 fusion gene, included; ALK/NPM1 fusion gene, included; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki 1; Anaplastic lymphoma kinase Ki1; Anaplastic lymphoma kinase p80 CD 246; CD246; CD246 antigen; EC 2.7.10.1 Ki 1; Ki1; NBLST3; Tcrz; TFG/ALK; ALK_HUMAN.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 细胞生物 染色质和核信号 神经生物学 信号转导 细胞凋亡 生长因子和** 激酶和磷酸酶 细胞膜受体
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-ALK (Tyr1604)抗体交叉反应 Human, Pig, Guinea Pig,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量174kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ALK around the phosphorylation site of Tyr1604:GH(p-Y)ED
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-ALK (Tyr1604)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), LK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011].
Function:
Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
Subunit:
Homodimer. Homodimerizes when bound to ligand. Interacts with FRS2, IRS1, MDK, PTN and SHC1. Interacts with CBL, PIK3R1 and PLCG1.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.
Tissue Specificity:
Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
Post-translational modifications:
Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation.
N-glycosylated.
phospho-ALK (Tyr1604)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-ALK (Tyr1604)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 NMDAR2B 谷氨酸受体2B抗体
合格 合格 合格 MEK2 丝裂原活化蛋白激酶激酶2抗体
合格 Thymosin Alpha-1 胸腺肽α1抗体
合格 c 胰岛素样生长因子I受体抗体
合格 FGFR1/CD331 碱性成纤维细胞生长因子受体1抗体
合格 PDGFRA 血小板源性生长因子受体A/PDGFRα抗体
合格 PDGF Receptor beta 血小板源性生长因子受体B/PDGFRβ抗体
合格 TERT 端粒酶反转录酶抗体
合格 合格 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon 14-3-3蛋白/14-3-3 α/β/γ/δ/ε亚型抗体
合格 HSP70 热休克蛋白70抗体
合格 EphB2 酪氨酸蛋白激酶受体B2抗体
合格 合格 脑源神经营养因子抗体
合格 RAR alpha 维甲酸受体RAR α/RAR α抗体
合格 Glucocorticoid receptor 糖皮质**受体抗体
合格 合格 Estrogen receptor alpha 雌**受体α抗体
合格 合格 Aquaporin 2 水通道蛋白-2抗体
合格 PKC beta 1 + 2 蛋白激酶C beta 1/2抗体
合格 Visfatin 内脂素/内脏脂肪素/前B细胞克隆增强因子1抗体
合格 合格 VEGF 血管内皮生长因子抗体
合格 Ovalbumin 鸡卵白蛋白/卵清蛋白抗体
合格 IASPP 肿瘤细胞凋亡ASPP家族的另一个成员抗体
合格 Neurofascin 神经束蛋白抗体
合格 合格 Insulin Receptor Beta 胰岛素受体β抗体
合格 TG 兔抗甲状腺球蛋白
合格 Bovine Serum Albumin 牛血清白蛋白抗体
合格 合格 Mesothelin 间皮素抗体
合格 Dnmt3b DNA甲基转移酶-3β抗体
合格 NMDAR2C 谷氨酸受体2C抗体（N端）
合格 合格 谷氨酸脱羧酶-65抗体
合格 MTLC 致癌基因抗体
合格 外周髓磷脂P0蛋白/P0蛋白抗体
合格 合格 CXCR3 细胞表面趋化因子受体3抗体
合格 Integrin beta 3 整合素β3抗体
合格 Amyloid Precursor Protein 淀粉样肽前体蛋白抗体（C端）
合格 合格 合格 Histone H3/HIST3H3 组蛋白H3抗体
合格 合格 GLUT2 葡萄糖转运蛋白2抗体
合格 PEX10 过氧化物酶体生物合成因子10抗体