phospho-AMPK alpha-1 (Thr183)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 5551R
英文名称phospho-AMPK alpha-1 (Thr183)
中文名称磷酸化腺苷单磷酸活化蛋白激酶α1抗体
别 名phospho-AMPK alpha-1(Thr198)(isoform 2); phospho-AMPK alpha-1(Thr183)(isoform 1); AMPK alpha 1; 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; AAPK1; acetyl CoA carboxylase kinase; AI194361 antibody AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha -1; AMPK 1; AMPK alpha 1 chain; AMPK antibody AMPK1; AMPKa1; AMPKalpha1;C130083N04Rik; cb116 antibody EC 2.7.11.1; HMG CoA reductase kinase; hormone sensitive lipase kinase; im:7154392 antibody kinase AMPK alpha1; MGC33776;MGC57364 antibody PRKAA 1; PRKAA1;Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; wu:fa94c10; AAPK1_HUMAN. AMPKα1; AMPK α1; AMPKα 1; AMPK α 1; AMPK AMPK-α1; AMPK-α-1; α-1;
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 **学 神经生物学 信号转导 转录调节因子 激酶和磷酸酶 Alzheimer's
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-AMPK alpha-1 (Thr183)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量64kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human AMPK alpha 1 around the phosphorylation site of Thr198(isoform 2)/Thr183(isoform 1):LR(p-T)SC
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-AMPK alpha-1 (Thr183)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].
Function:
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
Subunit:
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.
phospho-AMPK alpha-1 (Thr183)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-AMPK alpha-1 (Thr183)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 GAPDH(3E12)-Loading Control 3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)
合格 HPV16 E6 protein 人类**状瘤病毒16抗体
合格 CD31 血小板内皮细胞黏附分子1抗体
合格 S100B S100B蛋白
合格 CEA(B5) 癌胚抗原单克隆抗体（检测）
合格 Transferrin(1F10) 转铁蛋白单克隆抗体
合格 His tag 聚组氨酸抗体标签单克隆抗体
合格 GAPDH-Loading Control 3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)
合格 合格 Beta tubulin(Loading Control) 微管蛋白β tubulin/Tubulin β单克隆抗体（内参抗体）
合格 合格 PCNA 增殖细胞核抗原单克隆抗体
合格 beta-Actin β-肌动蛋白/β-Actin单克隆抗体（内参抗体）
合格 合格 COX4 细胞色素c氧化酶IV亚型1单克隆抗体
合格 Lamin B 核纤层蛋白B单克隆抗体(细胞核膜标志物)
合格 beta I Tubulin 微管蛋白β1单克隆抗体
合格 合格 Histone H3/HIST3H3 组蛋白H3单克隆抗体
合格 HSP70 热休克蛋白-70单克隆抗体
合格 P53 P53单克隆抗体
合格 CK7 细胞角蛋白7单克隆抗体
合格 Cytokeratin 8 细胞角蛋白8单克隆抗体
合格 NSE 神经元特异性烯醇化酶单克隆抗体
合格 CK17 细胞角蛋白17单克隆抗体
合格 Histone H3 (di methyl K79) 甲基化组蛋白H3(di methyl K79)单克隆抗体
合格 OCT-1 胚胎干细胞关键蛋白1单克隆抗体
合格 Histone H3 (mono methyl K79) 单甲基组蛋白H3(mono methyl K79)单克隆抗体
合格 CK18 细胞角蛋白18单克隆抗体
合格 CK18 细胞角蛋白18单克隆抗体
合格 Histone H3(di methyl K27) 甲基化组蛋白H3(di methyl K27)单克隆抗体
合格 NFKB p65 细胞核因子/k基因结合核因子单克隆抗体
合格 Histone H3(di methyl K79) 甲基化组蛋白H3(di methyl K79)单克隆抗体
合格 Collagen III III型胶原单克隆抗体
合格 HSP90 beta 热休克蛋白90β/HSP90 β 单克隆抗体
合格 Vimentin 波形蛋白单克隆抗体
合格 TUBB3 微管蛋白β3单克隆抗体
合格 合格 alpha smooth muscle Actin 肌动蛋白α/α-SMA/α Actin抗体
合格 alpha smooth muscle Actin 肌动蛋白α/α-SMA/α Actin抗体
合格 Caspase 8 半胱氨酸蛋白酶8单克隆抗体
合格 GAP43 神经生长相关蛋白43单克隆抗体