phospho-Androgen Receptor (Ser213)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 5191R
英文名称phospho-Androgen Receptor (Ser213)
中文名称磷酸化雄**受体抗体
别 名Androgen Receptor (phospho S213); Androgen Receptor (Phospho-Ser213); Androgen Receptor (phospho Ser213); p-Androgen Receptor (Ser213); ANDR_HUMAN; HYSP1; AIS; Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease); AR; DHTR; Dihydro Testosterone Receptor; Dihydrotestosterone receptor; HUMARA; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; Spinal and bulbar muscular atrophy; TFM.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 **学
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-Androgen Receptor (Ser213)抗体交叉反应 Human,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量101kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser213 [ER(p-S)GA]:ER(p-S)GA
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-Androgen Receptor (Ser213)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Function:
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. [ENZYME REGULATION] AIM-100 (4-amino-5,6-biaryl-furo[2,3-d]pyrimidine) suppresses TNK2-mediated phosphorylation at Tyr-267. Inhibits the binding of the Tyr-267 phosphorylated form to androgen-responsive enhancers (AREs) and its transcriptional activity.
Subunit:
Binds DNA as a homodimer. Part of a ternary complex containing AR, EFCAB6/DJBP and PARK7. Interacts with HIPK3 and NR0B2 in the presence of androgen. The ligand binding domain interacts with KAT7/HBO1 in the presence of dihydrotestosterone. Interacts with EFCAB6/DJBP, PELP1, PQBP1, RANBP9, RBAK, SPDEF, SRA1, TGFB1I1, ZNF318 and RREB1. Interacts with ZMIZ1/ZIMP10 and ZMIZ2/ZMIP7 which both enhance its transactivation activity. Interacts with SLC30A9 and RAD54L2/ARIP4. Interacts via the ligand-binding domain with LXXLL and FXXLF motifs from NCOA1, NCOA2, NCOA3, NCOA4 and MAGEA11. The AR N-terminal poly-Gln region binds Ran resulting in enhancement of AR-mediated transactivation. Ran-binding decreases as the poly-Gln length increases. Interacts with HIP1 (via coiled coil domain). Interacts (via ligand-binding domain) with TRIM68. Interacts with TNK2. Interacts with USP26. Interacts with RNF6. Interacts (regulated by RNF6 probably through polyubiquitination) with RNF14; regulates AR transcriptional activity. Interacts with PRMT2 and TRIM24. Interacts with GNB2L1/RACK1. Interacts with RANBP10; this interaction enhances dihydrotestosterone-induced AR transcriptional activity. Interacts with PRPF6 in a hormone-independent way; this interaction enhances dihydrotestosterone-induced AR transcriptional activity. Interacts with STK4/MST1. Interacts with ZIPK/DAPK3. Interacts with LPXN. Interacts with MAK. Part of a complex containing AR, MAK and NCOA3.
Subcellular Location:
Nucleus. Cytoplasm. Note=Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of GNB2L1.
phospho-Androgen Receptor (Ser213)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-Androgen Receptor (Ser213)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 RNF61/MKRN1 环指蛋白61抗体
合格 IGJ **球蛋白j链抗体
合格 ANKRD42 锚蛋白重复结构域蛋白42抗体
合格 CARKD 碳水化合物激酶结构域蛋白质抗体
合格 CC85C 卷曲螺旋域蛋白质85C抗体
合格 TSPAN6 四分子交联体6抗体（四旋蛋白）
合格 TSPAN13 四分子交联体13抗体（四旋蛋白）
合格 TSPAN20 四分子交联体20/四旋蛋白抗体
合格 TXNDC9 胚胎干细胞相关蛋白TXNDC9抗体
合格 HSPB7 热休克蛋白β7抗体
合格 GERP 环指蛋白27抗体
合格 TET2 甲基双加氧酶TET2抗体
合格 5 MethylCytosine 5甲基胞嘧啶抗体
合格 合格 SIRT5 沉默调节蛋白5抗体
合格 HOPX/LAGY 肺癌相关蛋白Y抗体
合格 VANGL2 神经管畸形相关蛋白VANGL2抗体
合格 MESP1 心血管发育中胚层相关蛋白1抗体
合格 NFAT5 活化T细胞核因子5蛋白抗体
合格 CNN2 钙结合蛋白Calponin 2抗体
合格 合格 SARP1 分泌细胞凋亡相关蛋白1抗体
合格 C5orf35 5号染色体开放阅读框35抗体
合格 合格 C5orf51 5号染色体开放阅读框51抗体
合格 合格 C9ORF43 9号染色体开放阅读框43抗体
合格 C9orf114 9号染色体开放阅读框114抗体
合格 C9orf152 9号染色体开放阅读框152抗体
合格 Factor XIIa heavy chain 凝血因子12重链抗体
合格 Kininogen-1 light chain 高分子量激肽原轻链抗体
合格 alpha 2 Macroglobulin α2巨球蛋白(α-2M)抗体
合格 Proteinase-activated receptor 4/PAR4 蛋白酶激活受体4抗体
合格 PI14 蛋白酶抑制剂14抗体
合格 ALAS-E 5-氨基乙酰丙酸合酶1抗体
合格 Mitoferrin 1 线粒体铁转运蛋白1抗体
合格 合格 C6orf64 6号染色体开放阅读框64抗体
合格 C6orf192 8号染色体开放阅读框192抗体
合格 合格 TSBP 睾丸特异蛋白抗体
合格 C6orf120 6号染色体开放阅读框120抗体