phospho-Androgen Receptor (Ser515)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 12471R
英文名称phospho-Androgen Receptor (Ser515)
中文名称磷酸化雄**受体抗体
别 名Androgen Receptor (phospho S515); Androgen Receptor (Phospho-Ser515); Androgen Receptor (phospho Ser515); p-Androgen Receptor (Ser515); ANDR_HUMAN; HYSP1; AIS; Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease); AR; DHTR; Dihydro Testosterone Receptor; Dihydrotestosterone receptor; HUMARA; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; Spinal and bulbar muscular atrophy; TFM.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 细胞生物 发育生物学 染色质和核信号 信号转导 表观遗传学
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-Androgen Receptor (Ser515)抗体交叉反应 Human,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量99kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser515 :YP(p-S)PT
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-Androgen Receptor (Ser515)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Function:
Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3.
Subunit:
Binds DNA as a homodimer. Part of a ternary complex containing AR, EFCAB6/DJBP and PARK7. Interacts with HIPK3 and NR0B2 in the presence of androgen. The ligand binding domain interacts with KAT7/HBO1 in the presence of dihydrotestosterone. Interacts with EFCAB6/DJBP, PELP1, PQBP1, RANBP9, RBAK, SPDEF, SRA1, TGFB1I1, ZNF318 and RREB1. Interacts with ZMIZ1/ZIMP10 and ZMIZ2/ZMIP7 which both enhance its transactivation activity. Interacts with SLC30A9 and RAD54L2/ARIP4. Interacts via the ligand-binding domain with LXXLL and FXXLF motifs from NCOA1, NCOA2, NCOA3, NCOA4 and MAGEA11. The AR N-terminal poly-Gln region binds Ran resulting in enhancement of AR-mediated transactivation. Ran-binding decreases as the poly-Gln length increases. Interacts with HIP1 (via coiled coil domain). Interacts (via ligand-binding domain) with TRIM68. Interacts with TNK2. Interacts with USP26. Interacts with RNF6. Interacts (regulated by RNF6 probably through polyubiquitination) with RNF14; regulates AR transcriptional activity. Interacts with PRMT2 and TRIM24. Interacts with GNB2L1/RACK1. Interacts with RANBP10; this interaction enhances dihydrotestosterone-induced AR transcriptional activity. Interacts with PRPF6 in a hormone-independent way; this interaction enhances dihydrotestosterone-induced AR transcriptional activity. Interacts with STK4/MST1. Interacts with ZIPK/DAPK3. Interacts with LPXN. Interacts with MAK. Part of a complex containing AR, MAK and NCOA3.
Subcellular Location:
Nucleus. Cytoplasm. Predominantly cytoplasmic in unliganded form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unliganded form in the presence of GNB2L1.
Tissue Specificity:
Isoform 2 is mainly expressed in heart and skeletal muscle.
phospho-Androgen Receptor (Ser515)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-Androgen Receptor (Ser515)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 IGSF12 **球蛋白超家族成员12抗体
合格 合格 IGSF21 **球蛋白超家族成员21抗体
合格 GDE3 促进成骨细胞分化因子/GDPD2抗体
合格 SERPINB11 丝氨酸蛋白酶抑制剂B11抗体
合格 SERPINB12 丝氨酸蛋白酶抑制剂B12抗体
合格 CST8 胱抑素8/半胱氨酸蛋白酶抑制剂8抗体
合格 C1orf149 1号染色体开放阅读框149抗体
合格 C1orf187/Draxin 1号染色体开放阅读框187抗体
合格 C1orf183 1号染色体开放阅读框183抗体
合格 RNF14 环指蛋白14抗体
合格 合格 RNF156 环指蛋白156抗体
合格 RNF35 环指蛋白35抗体
合格 RNF24 环指蛋白24抗体
合格 RNF25 环指蛋白25抗体
合格 RNF21 环指蛋白21抗体
合格 RNF123 环指蛋白123抗体
合格 RNF128 环指蛋白128抗体
合格 RNF133 环指蛋白133抗体
合格 RNF138 环指蛋白138抗体
合格 RNF113B 环指蛋白113B抗体
合格 合格 RNF150 环指蛋白150抗体
合格 RNF169 环指蛋白169抗体
合格 RNF170 环指蛋白170抗体
合格 合格 RNF186 环指蛋白186抗体
合格 phospho-IRF3 (Ser386) 磷酸化干扰素调节因子3抗体
合格 SLC47A1 溶质载体蛋白家族47成员A1抗体
合格 MUL1 E3泛素连接酶MUL1抗体
合格 合格 BTBD1 BTB/POZ结构域蛋白1（丙型肝炎病毒的NS5A反转录蛋白8）抗体
合格 ANKRD17 锚蛋白重复结构域蛋白17抗体
合格 ARHGAP32 Rho GTP酶激活蛋白32抗体
合格 SASS6 纺锤体组装异常蛋白6抗体
合格 NEK9 丝氨酸/苏氨酸蛋白激酶NEK9抗体
合格 合格 LRCH1 富含亮氨酸重复家庭蛋白1抗体
合格 LRCH3 富含亮氨酸重复家庭蛋白3抗体
合格 合格 MARCH9 膜相关环指蛋白9抗体
合格 PSMC2/Proteasome 19S S7 蛋白酶体PRS7抗体
合格 合格 PSMA7 蛋白酶体PSMα7抗体
合格 Proteasome 20S beta 6 蛋白酶体PSMβ6抗体
合格 Proteasome 26S S2 肿瘤坏死因子受体相关蛋白2抗体
合格 PSMD8 蛋白酶调解因子8抗体
合格 PSMD7 蛋白酶调解因子7抗体