phospho-ASK1 (Thr845)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 3031R
英文名称phospho-ASK1 (Thr845)
中文名称磷酸化细胞凋亡信号调节激酶1抗体
别 名ASK1 (phospho Thr845); Mitogen-activated protein kinase kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Apoptosis signal-regulating kinase 1; ASK-1; MAP3K5; ASK1; MAPKKK5; MEKK5; M3K5_HUMAN.
JournalPMIDIFApplicationPancreas (2014)254266133.0080WB
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-ASK1 (Thr845)抗体交叉反应 Human, Mouse, Rat,
产物应用WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg /test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量155kDa
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ASK1 around the phosphorylation site of Thr845:TE(p-T)FT
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-ASK1 (Thr845)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Function:
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
Subunit:
Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Part of a cytoplasmic complex made of HIPK1, DAB2IP and MAP3K5 in response to TNF. This complex formation promotes MAP3K5-JNK activation and subsequent apoptosis. Interacts with SOCS1 which recognizes phosphorylation of Tyr-718 and induces MAP3K5/ASK1 degradation in endothelial cells. Interacts with the 14-3-3 family proteins such as YWHAB, YWHAE, YWHAQ, YWHAH, YWHAZ and SFN. Interacts with ARRB2, BIRC2, DAB2IP, IGF1R, MAP3K6/ASK2, PGAM5, PIM1, PPP5C, SOCS1, STUB1, TRAF2, TRAF6 and TXN. Interacts with ERN1 in a TRAF2-dependent manner. Interacts with calcineurin subunit PPP3R1 and with PPM1L (By similarity). Interacts (via N-terminus) with RAF1 and this interaction inhibits the proapoptotic function of MAP3K5. Interacts with DAB2IP (via N-terminus C2 domain); the interaction occurs in a TNF-alpha-dependent manner.
Subcellular Location:
Cytoplasm. Endoplasmic reticulum. Note=Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.
Tissue Specificity:
Abundantly expressed in heart and pancreas.
Post-translational modifications:
Phosphorylated at Thr-838 through autophosphorylation and by MAP3K6/ASK2 which leads to activation. Thr-838 is dephosphorylated by PPP5C. Ser-83 and Ser-1033 are inactivating phosphorylation sites, the former of which is phosphorylated by AKT1 and AKT2. Phosphorylated at Ser-966 which induces association of MAP3K5/ASK1 with the 14-3-3 family proteins and suppresses MAP3K5/ASK1 activity. Calcineurin (CN) dephosphorylates this site. Also dephosphorylated and activated by PGAM5.
Ubiquitinated. Tumor necrosis factor (TNF) induces TNFR2-dependent ubiquitination leading to proteasomal degradation.
Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
SWISS:
O35099
Gene ID:
4217
phospho-ASK1 (Thr845)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-ASK1 (Thr845)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 KLHL11 Kelch样蛋白11抗体
合格 CABC1 伴侣蛋白bc1同源复合体抗体
合格 合格 AGFG2 HIV-1病毒复制结合蛋白2抗体
合格 ARRDC1 抑制蛋白结构域蛋白1抗体
合格 CCDC94 卷曲螺旋结构域蛋白94抗体
合格 CCDC124 卷曲螺旋结构域蛋白124抗体
合格 CCDC82 卷曲螺旋结构域蛋白82抗体
合格 CCDC136 鼻咽癌相关蛋白6抗体
合格 CCDC114 卷曲螺旋结构域蛋白114抗体
合格 CCDC34 卷曲螺旋结构域蛋白34抗体
合格 合格 CCDC90B 卷曲螺旋结构域蛋白90B抗体
合格 CCDC104 卷曲螺旋结构域蛋白104抗体
合格 CCDC129 卷曲螺旋结构域蛋白129抗体
合格 UMPS 尿苷磷酸合成酶抗体
合格 CCDC116 卷曲螺旋结构域蛋白116抗体
合格 CCDC117 卷曲螺旋结构域蛋白117抗体
合格 CCDC144NL 卷曲螺旋结构域蛋白144NL抗体
合格 CCDC146 卷曲螺旋结构域蛋白146抗体
合格 CCDC147 卷曲螺旋结构域蛋白147抗体
合格 CCDC148 卷曲螺旋结构域蛋白148抗体
合格 Zona pellucida glycoprotein 2 卵母细胞透明带糖蛋白2抗体
合格 CCDC160 卷曲螺旋结构域蛋白160抗体
合格 CCDC42 卷曲螺旋结构域蛋白42抗体
合格 CCDC46 卷曲螺旋结构域蛋白46抗体
合格 CCDC47 卷曲螺旋结构域蛋白47抗体
合格 CCDC54 卷曲螺旋结构域蛋白54抗体
合格 合格 CCDC83 卷曲螺旋结构域蛋白83抗体
合格 CCDC87 卷曲螺旋结构域蛋白87抗体
合格 FAM36A FAM36A蛋白抗体
合格 FAM134C FAM134C蛋白抗体
合格 CHD3 ATP依赖的解旋酶CHD3抗体
合格 RB1CC1/FIP200 RB1的诱导卷曲蛋白RB1CC1抗体
合格 CHCHD6 卷曲螺旋结构域蛋白CHCHD6抗体
合格 PHF8 组蛋白赖氨酸去甲基化酶PHF8抗体