phospho-ATF2 (Thr51)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 5172R
英文名称phospho-ATF2 (Thr51)
中文名称磷酸化活化复制因子2抗体
别 名ATF2(Phospho-Thr51); ATF2 (phospho Thr51); ATF2 (phospho T51); p-ATF2 (Thr51); p-ATF2 (T51); CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; CRE BP1; CRE-BP; CREB 2; CREB2; CREBP1; Cyclic AMP dependent transcription factor ATF 2; Cyclic AMP-responsive; ATF2_HUMAN.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 **学 染色质和核信号 信号转导 细胞凋亡 转录调节因子
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-ATF2 (Thr51)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量55kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ATF2 around the phosphorylation site of Thr51:DQ(p-T)PT
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-ATF2 (Thr51)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
Function:
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex.
Subunit:
Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.
Subcellular Location:
Nucleus.
Tissue Specificity:
Abundant expression seen in the brain.
Post-translational modifications:
Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4.
Similarity:
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger.
SWISS:
P15336
Gene ID:
1386
phospho-ATF2 (Thr51)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-ATF2 (Thr51)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Fibrinogen gamma chain 纤维蛋白原γ链抗体
合格 Fibrinopeptide B 纤维蛋白肽B/血纤肽B抗体
合格 PRC1 胞质分裂调控蛋白1抗体
合格 CDCREL 细胞周期调控相关蛋白1
合格 CCDC5 卷曲螺旋结构域蛋白5抗体
合格 合格 BMP9 骨形态发生蛋白9抗体
合格 CCDC69 卷曲螺旋结构域蛋白69抗体
合格 CCDC98 卷曲螺旋结构域蛋白98抗体
合格 CCDC90A 卷曲螺旋结构域蛋白90A抗体
合格 CD81 CD81抗体
合格 RNF74 环指蛋白74抗体（重组激活基因1）
合格 POMC 阿黑皮素原抗体
合格 合格 AKT1+2+3 蛋白激酶AKT1,2,3抗体
合格 LPAP **细胞磷蛋白磷酸酶相关蛋白抗体
合格 SEMA4D 跨膜蛋白SEMA4D抗体
合格 HLA DM 组织相容性复合体α抗体
合格 FOXN1 叉头蛋白N1抗体
合格 Mkl1 myocardin相关转录因子抗体
合格 Phospholipase C beta 1 磷酯酶Cβ1抗体
合格 CLCN3 氯离子通道蛋白3抗体
合格 合格 Neutrophil Elastase 中性粒细胞弹性蛋白酶ELANE抗体
合格 Wilms Tumor Protein 肾母细胞瘤蛋白抗体
合格 BRN3B 脑特定蛋白Brn3B抗体
合格 RuBisCO large subunit 核酮糖1,5二磷酸羧化酶/二磷酸核酮糖盐羧化酶大链抗体
合格 O-GlcNAc transferase O位N-乙酰葡萄糖胺OGT抗体
合格 PRAME 黑色素瘤优先表达抗原抗体
合格 合格 IFNAR2 干扰素α受体2抗体
合格 HSP10 热休克蛋白10/groES抗体
合格 EDIL3 表皮生长因子样重复折叠1结构域蛋白3抗体
合格 合格 Kidins220 220kDa蛋白激酶D相互作用蛋白抗体
合格 合格 EphB1+EphB2 酪氨酸蛋白激酶受体B1+B2抗体
合格 INPPL1 肌醇聚磷酸盐磷酸酶样蛋白1抗体
合格 DENTT 细胞分裂核仁蛋白1抗体
合格 FTO 脂肪堆积和肥胖相关蛋白抗体
合格 EMR1 表皮生长因子样**受体1抗体