phospho-AXL (Tyr698+Tyr702+Tyr703)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 5181R
英文名称phospho-AXL (Tyr698+Tyr702+Tyr703)
中文名称磷酸化粘附相关激酶抗体
别 名Adhesion related kinase; AI323647; Ark; Axl; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming gene; AXL transforming sequence/gene; EC 2.7.10.1; JTK11; Oncogene AXL; Tyro7; Tyrosine protein kinase receptor UFO; Tyrosine-protein kinase receptor UFO; UFO; UFO_HUMAN.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 信号转导 细胞周期蛋白 激酶和磷酸酶 细胞膜受体 细胞类型标志物
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-AXL (Tyr698+Tyr702+Tyr703)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量95kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human AXL around the phosphorylation site of Tyr698+Tyr702+Tyr703:KI(p-Y)NGD(p-Y)(p-Y)RQ <Cytoplasmic>
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-AXL (Tyr698+Tyr702+Tyr703)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors such as vitamin K dependent protein growth arrest specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia associated oncogene and also associated with colon cancer and melanoma.The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants (AXL1 and AXL2).
Function:
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol-3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.
Subunit:
Heterodimer and heterotetramer with ligand GAS6. Interacts with CBL, GRB2, LCK, NCK2, PIK3R1, PIK3R2, PIK3R3, PLCG1, SOCS1 and TENC1. Part a complex including AXL, TNK2 and GRB2, in which GRB2 promotes AXL recruitment by TNK2.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity:
Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
Post-translational modifications:
Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.
DISEASE:
Note=AXL and its ligand GAS6 are highly expressed in thyroid carcinoma tissues, and might thus be involved in thiroid tumorigenesis. Overexpression of AXL and its ligand was also detected in many other cancers such as myeloptoliferative disorders, prostatic carcinoma cells, or breast cancer.
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
SWISS:
P30530
Gene ID:
558
phospho-AXL (Tyr698+Tyr702+Tyr703)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-AXL (Tyr698+Tyr702+Tyr703)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Phospho-Paxillin(Tyr88) 磷酸化桩蛋白Paxillin抗体
合格 phospho-AMPK alpha-1 (Thr183) 磷酸化腺苷单磷酸活化蛋白激酶α1抗体
合格 phospho-PTPN7 (Ser246) 磷酸化非受体型蛋白酪氨酸磷酸酶7抗体
合格 合格 phospho-PKC delta (Ser645) 磷酸化蛋白激酶C亚性D型抗体
合格 phospho-PRKD3(Ser41) 磷酸化蛋白激酶C nu型抗体
合格 phospho-PTPN6(Tyr564) 磷酸化蛋白酪氨酸磷酸酶1C抗体
合格 phospho-PTPN6(Ser591) 磷酸化蛋白酪氨酸磷酸酶1C抗体
合格 phospho-PIK3R2 (Tyr467) 磷酸化磷脂酰肌醇激酶p85β抗体
合格 phospho-PRKCQ (Ser695) 磷酸化蛋白激酶C theta抗体
合格 合格 phospho-14-3-3 epsilon (Thr232) 磷酸化14-3-3E蛋白抗体
合格 合格 phospho-P53(Thr55) 磷酸化肿瘤抑制基因P53抗体
合格 phospho-TNIK (Ser764) 磷酸化TRAF2和NCK激酶相互作用蛋白抗体
合格 phospho-TERT(Ser1125) 磷酸化端粒酶逆转录酶抗体
合格 Phospho-Tuberin(Ser1418) 磷酸化结节性硬化蛋白抗体
合格 Phospho-Smad3 (Ser425) 磷酸化细胞信号转导分子SMAD3抗体
合格 phospho-Alpha synuclein (Tyr136) 磷酸化核突触蛋白α抗体
合格 phospho-Alpha synuclein(Ser129) 磷酸化核突触蛋白α抗体
合格 phospho-SYN1(Ser62+Ser67) 磷酸化神经突触素1抗体
合格 Phospho-SHP2 (Tyr81) 磷酸化蛋白酪氨酸磷酸酶2抗体
合格 phospho-SOX9(Ser181) 磷酸化转录因子SOX9蛋白抗体
合格 phospho-c-Raf(Ser339) 磷酸化原癌基因c-Raf抗体
合格 phospho-RAD9(Tyr28) 磷酸化细胞周期检查控制蛋白质抗体
合格 phospho-NFKB p65(Thr435) 磷酸化细胞核因子抗体
合格 phospho-NFKB p65(Thr254) 磷酸化细胞核因子抗体
合格 phospho-NFKB p65(Thr505) 磷酸化细胞核因子抗体
合格 phospho-RPS6KB1(Ser417) 磷酸化核糖体S6蛋白激酶抗体
合格 phospho-RPS6KB1(Ser427) 磷酸化核糖体S6蛋白激酶抗体
合格 phospho-RPS6KB1(Ser441+Thr444) 磷酸化核糖体S6蛋白激酶抗体
合格 phospho-ROCK2(Tyr256) 磷酸化Rho相关蛋白激酶2抗体
合格 phospho-RAD51(Thr309) 磷酸化Rad51抗体
合格 phospho-RPS6KA1(Ser352) 磷酸化丝氨酸/苏氨酸激酶p90RSK蛋白抗体
合格 phospho-p107(Ser975) 磷酸化视网膜母细胞瘤样蛋白p107抗体