phospho-B Raf (Thr598 + Ser601)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 12556R
英文名称phospho-B Raf (Thr598 + Ser601)
中文名称磷酸化叠-搁补蹿抗体
别 名B Raf (phospho T598 + S601); p-B Raf (phospho T598 + S601); 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; BRAF 1; Braf; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral (v-raf) oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; p94; RAFB 1; RAFB1; v raf murine sarcoma viral oncogene homolog B1; FLJ95109; BRAF_HUMAN.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 神经生物学 信号转导 细胞周期蛋白 转录调节因子 激酶和磷酸酶
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-B Raf (Thr598 + Ser601)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量94kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human B Raf around the phosphorylation site of Thr446 + Ser601:LA(p-T)VK(p-S)RW
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-B Raf (Thr598 + Ser601)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
The Raf kinases are important intermediates in signal transduction. Raf protein family members, including A Raf and B Raf, have intrinsic serine/threonine kinase activity. Interaction between Ras proteins and Raf proteins results in Raf-mediated phosphorylation and activation of MEK (also known as MAP kinase kinase). Defects in BRAF are involved in a wide range of cancers. B-Raf is a serine/threonine protein kinase that acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. 1 BRAF is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. BRAF acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones.
Function:
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
Subunit:
Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 (By similarity). Interacts with DGKH.
Subcellular Location:
Nucleus. Cytoplasm. Cell membrane.
Tissue Specificity:
Brain and testis.
Post-translational modifications:
Phosphorylation at Ser-365 by SGK1 inhibits its activity.
Methylation at Arg-671 decreases stability and kinase activity.
Ubiquitinated by RNF149; which leads to proteasomal degradation.
DISEASE:
Note=Defects in BRAF are found in a wide range of cancers. Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500]. Defects in BRAF are involved in lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.
Similarity:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
Gene ID:
673
phospho-B Raf (Thr598 + Ser601)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-B Raf (Thr598 + Ser601)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Phospho-MDM2(Thr218) 磷酸化双微体2癌基因抗体
合格 phospho-Tau (Ser416) 磷酸化微管相关蛋白抗体
合格 phospho-MBP(Thr232) 磷酸化髓鞘碱性蛋白抗体
合格 phospho-MBP(Tyr203) 磷酸化髓鞘碱性蛋白抗体
合格 合格 合格 phospho-P38 MAPK (Thr180) 磷酸化p38MAPK抗体
合格 phospho-p38 MAPK (Tyr323) 磷酸化p38MAPK抗体
合格 phospho-MEF2C(Ser387) 磷酸化肌细胞增强因子2C抗体
合格 phospho-MEF2C(Thr300) 磷酸化肌细胞增强因子2C抗体
合格 phospho-ERK5 (Ser496) 磷酸化细胞外信号调节激酶5抗体
合格 Phospho-MEF2A(Thr319) 磷酸化肌细胞增强因子2抗体
合格 phospho-ERK5 ( Ser731+Thr733) 磷酸化细胞外信号调节激酶5抗体
合格 合格 phospho-Ron(Tyr1238) 磷酸化原癌基因c-Met相关酪氨酸激酶抗体
合格 phospho-MAP4K4(Ser801) 磷酸化丝裂原活化蛋白激酶MAP4K4抗体
合格 phospho-MAP4K1(Ser171) 磷酸化造血祖细胞激酶抗体
合格 phospho-MAP3K7IP1 (Thr431) 磷酸化转化生长因子β活化激酶结合蛋白1抗体
合格 phospho-MEF2D(Ser180) 磷酸化肌细胞特异性增强因子2D抗体
合格 phospho-MEF2D(Ser444) 磷酸化肌细胞特异性增强因子2D抗体
合格 phospho-MAPKAPK5(Thr182) 磷酸化p38调节/激活蛋白激酶抗体
合格 合格 NFkB p105 细胞核因子p105/k基因结合核因子抗体
合格 合格 Phospho-NFKB1 (Ser903) 磷酸化细胞核因子p50/k基因结合核因子抗体
合格 Phospho-NFKB1 (Thr931) 磷酸化细胞核因子p50/k基因结合核因子抗体
合格 合格 Phospho-NFKB1 (Ser907) 磷酸化细胞核因子p50/k基因结合核因子抗体
合格 合格 Phospho-NFKB1 (Ser337) 磷酸化细胞核因子p50/k基因结合核因子抗体
合格 合格 Phospho-NFKB1 (Ser893) 磷酸化细胞核因子p50/k基因结合核因子抗体
合格 合格 合格 phospho-IKB alpha (Tyr42) 磷酸化IKB α抗体
合格 合格 phospho-IKB alpha (Tyr305) 磷酸化IKB alpha抗体
合格 合格 phospho-NDEL1(Ser242) 磷酸化中心粒蛋白Nudel抗体
合格 phospho-TrkB (Tyr515) 磷酸化酪氨酸激酶B抗体
合格 phospho-TrkB (Tyr705) 磷酸化酪氨酸激酶B抗体
合格 合格 FXR1 脆性X相关蛋白1抗体
合格 phospho-Kv2.1(Tyr128) 磷酸化钾通道蛋白DRK1抗体
合格 Phospho-PDPK1(Ser393) 磷酸化3磷酸肌醇依赖性蛋白激酶1抗体
合格 phospho-PIK3R1(Tyr368) 磷酸化磷脂酰肌醇激酶抗体