phospho-Bcl-xL (Thr47)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 12579R
英文名称phospho-Bcl-xL (Thr47)
中文名称磷酸化叠肠濒-虫尝蛋白抗体
别 名BCL2L1 (phospho T47); BCL2L1 (phospho Thr47); p-BCL2L1 (phospho T47); p-BCL2L1 (Thr47); p-BCL2L1(Thr115); Apoptosis regulator Bcl X; BclX; Bcl-X; Bcl 2 like 1; Bcl 2 like 1 protein; Bcl xL; BCL X/L; BCL XL/S; Bcl xS; Bcl2 like1; BCL2-related gene; BCL2-related protein, long isoform, included; BCLXL, included; BCL2-related protein, short isoform, included; BCLXS, included; BCL2L; BCL2L1; Bclx; DKFZp781P2092; MGC113803; MGC99998; B2CL1_HUMAN.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 新陈代谢 线粒体
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-Bcl-xL (Thr47)抗体交叉反应 Human, Mouse, Rat, Cow, Horse, Sheep, Guinea Pig,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量26kDa
细胞定位细胞核 细胞浆 细胞膜 线粒体
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human BCL2L1 around the phosphorylation site of Thr47:E(p-T)PS
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-Bcl-xL (Thr47)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008].
Function:
Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
Isoform Bcl-X(S) promotes apoptosis.
Subunit:
Homodimer. Isoform Bcl-X(L) forms heterodimers with BAX, BAK or BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Interacts with BCL2L11. Interacts with DMN1L; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with BAD and BBC3. Interacts (isoform Bcl-X(L)) with SIVA1 (isoform 1); the interaction inhibits the anti-apoptotic activity. Interacts with BECN1 and PGAM5. Interacts (isoform Bcl-X(L)) with BAX (isoform Sigma). Isoform Bcl-X(L) interacts with IKZF3. Interacts with HEBP2.
Subcellular Location:
Mitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, centrosome. Note=Mitochondrial membranes and perinuclear envelope. Localizes to the centrosome when phosphorylated at Ser-49.
Tissue Specificity:
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as brain.
Post-translational modifications:
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis.
Similarity:
Belongs to the Bcl-2 family.
SWISS:
Q07817
Gene ID:
598
phospho-Bcl-xL (Thr47)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-Bcl-xL (Thr47)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-Beta catenin (Tyr86) 磷酸化β 连环素蛋白抗体
合格 LATS2 肿瘤抑制基因LATS2抗体
合格 phospho-c-Abl (Tyr134) 磷酸化非受体酪氨酸激酶c-Abl抗体
合格 BACH1 乳腺癌易感基因相互作用蛋白1抗体
合格 合格 Cdc25B 细胞分裂周期蛋白25B抗体
合格 CTNNBIP1 β链接素相互作用蛋白1抗体
合格 CASC3 肿瘤易感候选基因3抗体
合格 ECT2 上皮细胞癌转化蛋白2抗体
合格 CRF 促肾上腺皮质**释放因子/促肾上皮质**释放**抗体
合格 合格 Myoglobin 肌红蛋白抗体
合格 IKBKE/IKKi/IKKe 核因子κB抑制蛋白E抗体
合格 IFNAR1 干扰素α受体抗体
合格 ASGPR1 唾液酸糖蛋白受体1抗体
合格 Exendin 4 GLP1类似蛋白Exendin4抗体
合格 MYL6 肌球蛋白轻链6抗体
合格 ERK4 细胞外信号调节激酶4抗体
合格 合格 合格 MAP4K1 造血祖细胞激酶1抗体
合格 MEF2D 肌细胞特异性增强因子2D抗体
合格 Glutamine synthetase 谷氨酰胺合成酶/谷氨酸氨连接酶抗体
合格 Keratan Sulfate 硫酸角质素抗体
合格 PAK6 P21蛋白激活的蛋白激酶6抗体
合格 PI 3 Kinase Class 3 磷脂酰肌醇激酶3催化亚单位3抗体
合格 PI 3 Kinase p85 beta 磷脂酰肌醇激酶p85β抗体
合格 PKC theta 蛋白激酶C theta抗体
合格 phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)
合格 TRIM25 雌**反应锌指蛋白抗体
合格 Synaptotagmin 1 突触结合蛋白1抗体
合格 SYT6 突触蛋白6抗体
合格 合格 合格 SOX9 转录因子SOX9蛋白抗体
合格 P2Y2 嘌呤ATP受体抗体
合格 p53R2 核苷酸还原酶M2B抗体
合格 合格 RPA32 复制因子A蛋白2
合格 RelB 转录因子RelB蛋白抗体