Phospho-CDK2 (Thr160)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 3483R
英文名称Phospho-CDK2 (Thr160)
中文名称磷酸化周期素依赖性激酶2抗体
别 名p-CDH2 (Thr160); CDH2 (phospho-Thr160); CDH2 (phospho-T160); Cdc2 related protein kinase; cdc2-related protein kinase; Cdk 2; CDK2; CDK2_HUMAN; Cell devision kinase 2; Cell division kinase 2; Cell division protein kinase 2; Cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; p33 protein kinase; p33(CDK2).
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 信号转导 细胞周期蛋白 转录调节因子 激酶和磷酸酶
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
Phospho-CDK2 (Thr160)抗体交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量33kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human CDK2 around the phosphorylation site of Thr160 [TY(p-T)HE]:TY(p-T)HE
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-CDK2 (Thr160)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq, Jul 2008].
Function:
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization.
Phospho-CDK2 (Thr160)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-CDK2 (Thr160)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 MED12/Trap230 甲状腺**受体相关蛋白复合物230抗体
合格 MED4 MED4蛋白抗体
合格 MED6 MED6蛋白抗体
合格 MEGF11 MEGF11蛋白抗体
合格 MEKK3 MAPK/ERK激酶3抗体
合格 phospho-MERTK (Tyr749 + Tyr753 + Tyr754) 磷酸化c-mer原癌基因酪氨酸激酶抗体
合格 MESDC2 中胚层分化蛋白2抗体
合格 MESP2 碱性螺旋环螺旋蛋白MESP2抗体
合格 合格 MEST 中胚层特异性转录同源蛋白MEST抗体
合格 PSAP 鞘脂激活蛋白原抗体
合格 Methylated Lysine (di methyl , mono methyl ) 赖氨酸甲基化抗体
合格 Securin 垂体肿瘤转化基因抗体
合格 MEX3D/RNF193 环指蛋白193抗体
合格 合格 RRP7A RRP7A蛋白抗体
合格 RTL1 哺乳动物反转录蛋白RTL1抗体
合格 MFRP 膜型卷曲相关蛋白MFRP抗体
合格 MIA3 黑色素瘤抑制活性蛋白3抗体
合格 MIF4GD MIF4GD蛋白抗体
合格 MLH3 错配修复蛋白3抗体
合格 MMACHC 甲基丙二酸尿症和高胱氨酸尿症C型蛋白同源抗体
合格 MMP27 基质金属蛋白酶27抗体
合格 合格 Myozenin 2 肌肉特异性蛋白MYOZ2抗体
合格 N myc interactor 致癌基因N myc相互作用因子抗体
合格 NACAD NACAD蛋白抗体
合格 NAGA α-半乳糖苷酶B抗体
合格 Nance-Horan Syndrome Protein 南斯-霍兰综合征蛋白抗体
合格 NAP1/C12orf30 12号染色体开放阅读框30抗体
合格 NAPE-PLD N酰基磷脂酰乙醇胺磷脂酶D抗体
合格 合格 NAPG 可溶性NSF附着蛋白γ-SNAP抗体
合格 NAPRT1 烟酸磷酸NAPRT1抗体
合格 NARFL NARFL蛋白抗体
合格 NARG1 NMDA受体调节1抗体