笔丑辞蝉辫丑辞-肠-碍颈迟(罢测谤721)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 3242R
英文名称笔丑辞蝉辫丑辞-肠-碍颈迟(罢测谤721)
中文名称磷酸化原癌基因肠-办颈迟抗体
别 名c-Kit (phospho Y721); c-Kit (phospho Tyr721); p-c-Kit (Tyr 721); C Kit; c-Kit; CD 117; CD117; CD117 antigen; KIT; KIT_HUMAN; Mast/stem cell growth factor receptor; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto oncogene c Kit; Proto oncogene tyrosine protein kinase Kit; Proto-oncogene c-Kit; SCF Receptor; SCFR; soluble KIT variant 1; Stem cell factor receptor; tyrosine protein kinase Kit; Tyrosine-protein kinase Kit; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 **学 信号转导 转录调节因子 激酶和磷酸酶
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
笔丑辞蝉辫丑辞-肠-碍颈迟(罢测谤721)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量145kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human c-Kit around the phosphorylation site of Tyr721:NE(p-Y)MD
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
笔丑辞蝉辫丑辞-肠-碍颈迟(罢测谤721)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
c-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.
Function:
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate.
Subunit:
Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC. Subcellular Location : Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Single-pass type I membrane protein. Isoform 3: Cytoplasm. Note=Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Single-pass type I membrane protein. Isoform 3: Cytoplasm. Note=Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
Tissue Specificity:
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
笔丑辞蝉辫丑辞-肠-碍颈迟(罢测谤721)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
笔丑辞蝉辫丑辞-肠-碍颈迟(罢测谤721)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 C21ORF13 21号染色体开放阅读框13抗体
合格 合格 合格 C21orf33 21号染色体开放阅读框33抗体
合格 C21orf7 21号染色体开放阅读框7抗体
合格 C21orf88 21号染色体开放阅读框88抗体
合格 C22orf13 22号染色体开放阅读框13抗体
合格 C22orf26 22号染色体开放阅读框26抗体
合格 合格 EpCAM 上皮细胞粘附分子抗体
合格 C22orf43 22号染色体开放阅读框43抗体
合格 C2CD4D C2CD4D蛋白抗体
合格 C2orf16 2号染色体开放阅读框16抗体
合格 合格 CD33 CD33抗体
合格 C2orf49 2号染色体开放阅读框49抗体
合格 C2orf53 2号染色体开放阅读框53抗体
合格 C2orf56 2号染色体开放阅读框56抗体
合格 C2orf65 2号染色体开放阅读框65抗体
合格 C2orf72 2号染色体开放阅读框72抗体
合格 C2orf88 2号染色体开放阅读框88抗体
合格 C3IP1 补体C3IP1抗体
合格 C3orf14 3号染色体开放阅读框14抗体
合格 C3orf25 3号染色体开放阅读框25抗体
合格 C3orf38 3号染色体开放阅读框38抗体
合格 C3orf58 3号染色体开放阅读框58抗体
合格 C4orf29 4号染色体开放阅读框29抗体
合格 C4orf52 4号染色体开放阅读框52抗体
合格 E cadherin 上皮钙粘附分子抗体
合格 C5orf22 5号染色体开放阅读框22抗体
合格 C5orf24 5号染色体开放阅读框24抗体
合格 C5orf34 5号染色体开放阅读框34抗体
合格 C5ORF42 5号染色体开放阅读框42抗体
合格 C5orf48 5号染色体开放阅读框48抗体
合格 C5orf49 5号染色体开放阅读框49抗体
合格 合格 CD7 CD7抗体
合格 C5orf54 5号染色体开放阅读框54抗体
合格 C5orf55 5号染色体开放阅读框55抗体
合格 C6orf132 6号染色体开放阅读框132抗体
合格 C6orf136 6号染色体开放阅读框136抗体
合格 C6orf145 6号染色体开放阅读框145抗体
合格 C6ORF173 6号染色体开放阅读框173抗体