Phospho-CRMP2 (Thr514)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 3068R
英文名称Phospho-CRMP2 (Thr514)
中文名称磷酸化二氢嘧啶酶样2抗体
别 名CRMP2 (phospho T514); p-CRMP2 (phospho T514); CRMP2 (phospho T514); p-CRMP2 (phospho T514); CRMP2 (phospho T514); CRMP-2(phospho Thr514); p-CRMP2(Thr514); Collapsin response mediator protein 2; Collapsin response mediator protein hCRMP 2; CRAM; CRMP 2; CRMP-2; CRMP2; DHPRP 2; DHPRP2; Dihydr pyrimidinase 2; Dihydropyrimidinase 2; Dihydropyrimidinase like 2; Dihydropyrimidinase like 2 long form; Dihydropyrimidinase related protein 2; Dihydropyrimidinase-related protein 2; DPYL 2; DPYL2; DPYL3_HUMAN; DPYSL 2; Dpysl2; DRP 2; DRP-2; DRP2; Musunc 33; Musunc33; N2A3; TOAD 64; TOAD64; ULIP 2 protein; ULIP-2; Ulip2; Unc-33-like phosphoprotein 2.
说 明 书100ul
产物类型磷酸化抗体
研究领域细胞生物 **学 发育生物学 神经生物学 生长因子和**
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
Phospho-CRMP2 (Thr514)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量62kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human CRMP-2 around the phosphorylation site of Thr514:TV(p-T)PA
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-CRMP2 (Thr514)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The CRMPs appear to play a complex role in axon growth as well as microtubule dynamics and axon induction. CRMPs localize to the lamellipodia and filopodia of axonal growth cones, suggesting a role in axon guidance. Moreover, CRMP2 is upregulated after axotomy, and appears to increase the formation of axon-type processes from hippocampal neurons. CRMP2 has been reported to bind tubulin dimers directly and modulate microtubule assembly. CRMPs have also been implicated in the pathogenesis of a paraneoplastic neurologic syndrome. Interaction studies have implicated phospholipase D2 (PLD2), the cytosolic tyrosine kinase Fes, and intersectin in CRMP function. Hyperphosphorylation of CRMP2 is an early event in the progression of Alzheimer's disease.
Function:
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).
Subunit:
Homotetramer, and heterotetramer with CRMP1, DPYSL2, DPYSL4 or DPYSL5 (By similarity). Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin (By similarity).
Subcellular Location:
Cytoplasm. Cell projection, growth cone. Note=Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone.
Tissue Specificity:
Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.
Post-translational modifications:
Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3.
Similarity:
Belongs to the DHOase family.
Gene ID:
1808
Phospho-CRMP2 (Thr514)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-CRMP2 (Thr514)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
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合格 PEX11B 过氧化物酶体生物合成因子11B抗体
合格 PEX5 过氧化物酶体生物合成因子5抗体
合格 PEX6 过氧化物酶体生物合成因子6抗体
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合格 DAP12 自然杀伤激活受体相关蛋白DAP12抗体
合格 PFKFB3/PFK2 果糖-2,6-二磷酸酶3/磷酸果糖激酶2抗体
合格 SMCR7 SMCR7蛋白抗体
合格 SMG5 SMG5蛋白抗体
合格 SMPDL3B 酸性鞘磷脂酶样磷酸二酯酶3B抗体
合格 SMUG1 SMUG1蛋白抗体
合格 SMYD1 SMYD1蛋白抗体
合格 SMYD5 SMYD5蛋白抗体
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合格 SNRK SNF相关丝氨酸/苏氨酸蛋白激酶抗体
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合格 TMPRSS11D 跨膜丝氨酸蛋白酶11D抗体
合格 TMX3 硫氧还蛋白相关跨膜蛋白3抗体
合格 VAC14 VAC14蛋白抗体
合格 Macrophage Inflammatory Protein 3 alpha 巨噬细胞炎性蛋白MIP-3a抗体
合格 PKNOX1 同源盒蛋白PKNOX1抗体
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合格 phospho-DRP1 (Ser616) 磷酸化动力相关蛋白1抗体
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合格 CK16 细胞角蛋白16抗体
合格 合格 合格 phospho-IRS1 (Ser616) 磷酸化胰岛素受体底物1抗体
合格 PLD3 胆碱磷酸酯酶3抗体
合格 HTRA2 线粒体丝氨酸蛋白酶抗体
合格 PLEKHA1 含普列克底物蛋白家族A成员1抗体
合格 合格 E-Selectin E选择素抗体
合格 PMM2 磷酸甘露糖变位酶2抗体
合格 PNMA5 类肿瘤性抗原MA5抗体
合格 PNPLA3 脂肪营养素PNPLA3抗体
合格 Integrin alpha E 整合素αE抗体
合格 PODNL1 PODN样蛋白1抗体
合格 POFUT2 蛋白O岩藻糖基转移酶2抗体