phospho-EPH A3+A4+A5 ( Tyr779)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 7555R
英文名称phospho-EPH A3+A4+A5 ( Tyr779)
中文名称磷酸化内皮细胞受体蛋白酪氨酸激酶础3+础4+础5抗体
别 名EPH A3+A4+A5 (phospho Y779 + Y779 + Y833); EPH A3+A4+A5 (phospho Y779); p-EPH A3+A4+A5 (phospho Y779); CEK7; EHK 1; EHK1; EK4; EK7; EK8; EPH homology kinase 1; EPH like kinase 4; EPH like kinase 7; EPH like kinase 8; Eph like tyrosine kinase 1; EPH receptor A3; EPH receptor A4; EPH receptor A5; Ephrin type A receptor 3; Ephrin type A receptor 4; Ephrin type A receptor 5; ETK; ETK1; HEK; HEK4; HEK7; HEK8; Human embryo kinase 1; Receptor protein tyrosine kinase HEK7; Receptor protein tyrosine kinase HEK8; SEK; TYRO1; TYRO1 protein tyrosine kinase; TYRO4; TYRO4 protein tyrosine kinase; Tyrosine protein kinase receptor EHK 1; Tyrosine protein kinase receptor ETK1; Tyrosine protein kinase receptor SEK; Tyrosine protein kinase TYRO1; EPHA3_HUMAN; EPHA4_HUMAN; EPHA5_HUMAN.
说 明 书100ul
产物类型磷酸化抗体
研究领域细胞生物 神经生物学 信号转导 激酶和磷酸酶 细胞膜受体
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-EPH A3+A4+A5 ( Tyr779)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量108kDa
细胞定位细胞膜 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human around the phosphorylation site of EPH 3 Tyr779:AA(p-Y)TT
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-EPH A3+A4+A5 ( Tyr779)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
EphA3 is a member of the Ephrin (Eph) family. The Ephrins and Eph-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the Ephrin A (EphA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the Ephrin B (EphB) class, which are transmembrane proteins. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats.
Function:
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
Subunit:
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding by ADAM10 which regulates the EFNA5-EPHA3 complex internalization and function. Interacts with NCK1 (via SH2 domain); mediates EFNA5-EPHA3 signaling (By similarity). Interacts (phosphorylated) with PTPN1; dephosphorylates EPHA3 and may regulate its trafficking and function. Interacts (phosphorylated) with CRK; mediates EFNA5-EPHA3 signaling through RHOA GTPase activation.
Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein.
Isoform 2: Secreted.
Tissue Specificity:
Widely expressed. Highest level in placenta.
Post-translational modifications:
Autophosphorylates upon activation by EFNA5. Phosphorylation on Tyr-602 mediates interaction with NCK1. Dephosphorylated by PTPN1.
DISEASE:
Defects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500].
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
SWISS:
P29320
Gene ID:
2042
phospho-EPH A3+A4+A5 ( Tyr779)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-EPH A3+A4+A5 ( Tyr779)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 MUL1 E3泛素连接酶MUL1抗体
合格 合格 BTBD1 BTB/POZ结构域蛋白1（丙型肝炎病毒的NS5A反转录蛋白8）抗体
合格 ANKRD17 锚蛋白重复结构域蛋白17抗体
合格 ARHGAP32 Rho GTP酶激活蛋白32抗体
合格 SASS6 纺锤体组装异常蛋白6抗体
合格 NEK9 丝氨酸/苏氨酸蛋白激酶NEK9抗体
合格 合格 LRCH1 富含亮氨酸重复家庭蛋白1抗体
合格 LRCH3 富含亮氨酸重复家庭蛋白3抗体
合格 合格 MARCH9 膜相关环指蛋白9抗体
合格 PSMC2/Proteasome 19S S7 蛋白酶体PRS7抗体
合格 合格 PSMA7 蛋白酶体PSMα7抗体
合格 Proteasome 20S beta 6 蛋白酶体PSMβ6抗体
合格 Proteasome 26S S2 肿瘤坏死因子受体相关蛋白2抗体
合格 PSMD8 蛋白酶调解因子8抗体
合格 PSMD7 蛋白酶调解因子7抗体
合格 RNF61/MKRN1 环指蛋白61抗体
合格 IGJ **球蛋白j链抗体
合格 ANKRD42 锚蛋白重复结构域蛋白42抗体
合格 CARKD 碳水化合物激酶结构域蛋白质抗体
合格 CC85C 卷曲螺旋域蛋白质85C抗体
合格 TSPAN6 四分子交联体6抗体（四旋蛋白）
合格 TSPAN13 四分子交联体13抗体（四旋蛋白）
合格 TSPAN20 四分子交联体20/四旋蛋白抗体
合格 TXNDC9 胚胎干细胞相关蛋白TXNDC9抗体
合格 HSPB7 热休克蛋白β7抗体
合格 GERP 环指蛋白27抗体
合格 TET2 甲基双加氧酶TET2抗体
合格 5 MethylCytosine 5甲基胞嘧啶抗体
合格 合格 SIRT5 沉默调节蛋白5抗体
合格 HOPX/LAGY 肺癌相关蛋白Y抗体
合格 VANGL2 神经管畸形相关蛋白VANGL2抗体
合格 MESP1 心血管发育中胚层相关蛋白1抗体
合格 NFAT5 活化T细胞核因子5蛋白抗体
合格 CNN2 钙结合蛋白Calponin 2抗体
合格 合格 SARP1 分泌细胞凋亡相关蛋白1抗体
合格 C5orf35 5号染色体开放阅读框35抗体
合格 合格 C5orf51 5号染色体开放阅读框51抗体
合格 合格 C9ORF43 9号染色体开放阅读框43抗体
合格 C9orf114 9号染色体开放阅读框114抗体
合格 C9orf152 9号染色体开放阅读框152抗体
合格 Factor XIIa heavy chain 凝血因子12重链抗体
合格 Kininogen-1 light chain 高分子量激肽原轻链抗体