Phospho-FoxO1 (Ser256)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 3142R
英文名称Phospho-FoxO1 (Ser256)
中文名称磷酸化叉头蛋白家族1抗体
别 名FOXO1A (phospho S256); p-FOXO1A (phospho S256); FKHR(Phospho-Ser256); Forkhead box protein O1; Afxh; AI876417; FKHR; Fkhr1; Foxo1a; Forkhead; FKH 1; FKH1; FKH1; FKHR; FKHR; Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma); Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma); Forkhead box O1; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; Forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOXO1; FOXO1; FOXO1_HUMAN; FOXO1A.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 **学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
Phospho-FoxO1 (Ser256)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
产物应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量72kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human FOXO1 around the phosphorylation site of Ser256 [AA(p-S)MD]:AA(p-S)MD
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-FoxO1 (Ser256)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq].
Function:
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. Triggers death of postmitotic neurons when phosphorylated by CDK1. Activates transcription of PMAIP1.
Subunit:
Interacts with LRPPRC. Interacts with SIRT1 and this interaction requires the presence of KRIT1. Interacts with NLK. Binds to CDK1 and 14-3-3 proteins.
Subcellular Location:
Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus. Translocates to the nucleus upon oxidative stress induced phosphorylation at Ser-212 by STK4/MST1. Translocates to the nucleus upon phosphorylation of Thr-24, Ser-256 and Ser-322 by SGK1.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Phosphorylated by AKT1; insulin-induced. Phosphorylated by NLK, which inhibits transcriptional activity and promotes nuclear export (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation of Ser-249 by CDK1 disrupts 14-3-3 proteins binding and thereby promotes FOXO1 nuclear accumulation and subsequent transcription activation and cell death. Phosphorylated by STK4/MST1 on Ser-212 upon oxidative stress. Phosphorylated on Thr-24, Ser-256 and Ser-322 by SGK1 resulting in its translocation from the nucleus to the cytoplasm.
DISEASE:
Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
Similarity:
Contains 1 fork-head DNA-binding domain.
SWISS:
Q12778
Gene ID:
2308
Phospho-FoxO1 (Ser256)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-FoxO1 (Ser256)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Beta galactosidase β半乳糖苷酶抗体
合格 HOMER2 HOMER2抗体
合格 CRLF2 胸腺基质**细胞生成素受体抗体
合格 Mst2 蛋白激酶MST2抗体
合格 Caspase-8 subunit p18 半胱氨酸蛋白酶8抗体
合格 合格 AMH Muellerian缪勒管**抑制因子抗体
合格 Tissue factor/CD142 组织因子抗体
合格 Macrophage mannose receptor 1 巨噬细胞甘露糖受体抗体
合格 合格 NADPH oxidase 4 NADPH氧化酶4抗体
合格 合格 CD168 透明质酸介导细胞游走受体抗体
合格 IFITM1 干扰素诱导跨膜蛋白1抗体
合格 von Willebrand antigen 2 血管假性血友病因子/血管性血友病因子抗体
合格 合格 CD19 CD19抗体
合格 合格 CA153 乳腺癌相关抗原抗体
合格 Metabotropic Glutamate Receptor 2 促代谢型谷氨酸受体2抗体
合格 合格 CD133 造血干细胞抗原CD133抗体
合格 KCNMA1/BK channel 钙激活钾通道蛋白α1抗体
合格 合格 phospho-CD19 (Ser227) 磷酸化CD19抗体
合格 CD8 CD8抗体
合格 合格 CD8 alpha CD8抗体
合格 CD4 CD4抗体
合格 CD45 白细胞共同抗原抗体
合格 CD45 白细胞共同抗原抗体
合格 CD45 白细胞共同抗原抗体
合格 AFP 甲胎蛋白AFP抗体
合格 CD83 CD83抗体
合格 FHL1 骨骼肌蛋白FHL1抗体
合格 CD73 CD73抗体
合格 合格 Phospho-eIF2 alpha(Ser51) 磷酸化真核启动因子2α抗体
合格 合格 LC3B 自噬微管相关蛋白轻链β3抗体
合格 合格 GLUT1 葡萄糖转运蛋白1抗体
合格 Nephrin 肾小球细胞粘附分子受体抗体
合格 Complement C3dg fragment 补体C3d**段抗体
合格 IKK beta KB抑制蛋白激酶β抗体
合格 合格 GOLPH2 高尔基体膜蛋白GP73抗体
合格 Phospho-PPAR Gamma (ser273) 磷酸化过氧化酶活化增生受体γ抗体 PPARγ
合格 Keratan Sulfate 硫酸角质素抗体