辫丑辞蝉辫丑辞-骋3叠笔1(厂别谤232)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 5376R
英文名称辫丑辞蝉辫丑辞-骋3叠笔1(厂别谤232)
中文名称磷酸化Ras GTP酶活化蛋白结合蛋白1抗体
别 名G3BP (phospho S232); p-G3BP (phospho S232); G3BP-1(Phospho-Ser232); ATP dependent DNA helicase VIII; G3BP; G3BP1; GAP binding protein; GAP SH3 domain binding protein 1; HDH VIII; MGC111040; Ras GTPase activating protein binding protein 1; Ras GTPase activating protein SH3 domain binding protein; RasGAP associated endoribonuclease G3BP.
JournalPMIDIFApplicationJournal of Proteome Research (2014)245335055.0560WB
说 明 书100ul
产物类型磷酸化抗体
研究领域细胞生物 **学 染色质和核信号 表观遗传学
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
辫丑辞蝉辫丑辞-骋3叠笔1(厂别谤232)抗体交叉反应 Human, Pig, Cow, Rabbit,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量52-60kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human G3BP1 around the phosphorylation site of Ser232:SS(p-S)PA
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
辫丑辞蝉辫丑辞-骋3叠笔1(厂别谤232)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Function:
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
Subcellular Location:
Cytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization. Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
Similarity:
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.
SWISS:
Q13283
Gene ID:
10146
辫丑辞蝉辫丑辞-骋3叠笔1(厂别谤232)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
辫丑辞蝉辫丑辞-骋3叠笔1(厂别谤232)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 ATG1/ULK1 自噬相关蛋白1抗体
合格 WNK1 赖氨酸缺陷型蛋白激酶1抗体
合格 CYP11A1 细胞色素P450 11A1抗体
合格 合格 Caveolin-3 细胞质膜微囊蛋白-3抗体
合格 合格 EIF2S1/eIF2 alpha 真核启动因子2α抗体(eIFα2)
合格 合格 PPAR alpha α型-过氧化酶活化增生受体抗体
合格 Talin 踝蛋白抗体
合格 Phospho-Zyxin (Ser142+Ser143) 磷酸化斑联蛋白抗体
合格 Delta Opioid Receptor D型受体抗体
合格 PKC delta 蛋白激酶C亚性D型抗体
合格 合格 Synaptopodin 突触足蛋白抗体
合格 RACK1 受体激活蛋白激酶C1抗体
合格 HOXB4 HOXB4抗体
合格 FPRL1 脂氧素受体1抗体
合格 KCNA7 钾电压阀门通道混合器相关亚家族成员7抗体
合格 FLASH 半胱氨酸蛋白酶8相关蛋白2抗体
合格 NIPP1 核抑制蛋白磷酸酶1抗体
合格 Caspase-7 p20 半胱胺酸蛋白酶蛋白-7抗体
合格 Calmodulin 钙调节素/钙调蛋白/钙调素抗体
合格 SLC44A1 溶质转运蛋白家族44成员1抗体
合格 Brs3 蛙皮素受体3抗体
合格 BMP6 骨形态发生蛋白6抗体
合格 HSP22 热休克蛋白-22抗体
合格 PBR 外周苯二氮受体抗体
合格 RALDH2 视黄醛脱氢酶2型抗体
合格 COMP 软骨寡聚基质蛋白抗体
合格 Nox1 有丝分裂氧化酶1抗体
合格 合格 CD1d T**细胞CD1D抗体