Phospho-Histone H3 (Thr11)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 3188R
英文名称Phospho-Histone H3 (Thr11)
中文名称磷酸化组蛋白贬3抗体
别 名Histone H3 (phospho T11); p-Histone H3 (phospho T11); Phospho-Histone H3(Thr11); Histone H3(Phospho-Thr11); Histone H3(p-Thr11); Histone H3(Phospho-T11); H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; H31_TETTH; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 **学 染色质和核信号 信号转导 转录调节因子 表观遗传学
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
Phospho-Histone H3 (Thr11)抗体交叉反应 Human, Mouse, Rat, Pig, Cow, Rabbit, Fruit Fly,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量15kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human Histone H3 around the phosphorylation site of Thr11:KS(p-T)GG
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-Histone H3 (Thr11)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
Function:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination.
Subunit:
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with GCN5, whereby H3S10ph increases histone-protein interactions. Interacts with PDD1 and PDD3.
Subcellular Location:
Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC).
Post-translational modifications:
Phosphorylated to form H3S10ph. H3S10ph promotes subsequent H3K14ac formation by GCN5. H3S10ph is only found in the mitotically dividing MIC, but not in the amitotically dividing MAC. H3S10ph is correlated with chromosome condensation during mitotic or meiotic micronuclear divisions.
Acetylation of histone H3 leads to transcriptional activation. H3K14ac formation by GCN5 is promoted by H3S10ph. H3K9acK14ac is the preferred acetylated form of newly synthesized H3. Acetylation occurs almost exclusively in the MAC.
Methylated to form H3K4me. H3K4me is only found in the transcriptionally active MAC. Methylated to form H3K9me in developing MACs during conjugation, when genome-wide DNA elimination occurs. At this stage, H3K9me specifically occurs on DNA sequences being eliminated (IES), probably targeted by small scan RNAs (scnRNAs) bound to IES, and is required for efficient IES elimination. H3K9me is required for the interaction with the chromodomains of PDD1 and PDD3.
The full-length protein H3S (slow migrating) is converted to H3F (fast migrating) by proteolytic removal of the first 6 residues. H3F is unique to MIC, and processing seems to occur regularly each generation at a specific point in the cell cycle.
Similarity:
Belongs to the histone H3 family.
SWISS:
P68431
Gene ID:
8290
Phospho-Histone H3 (Thr11)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-Histone H3 (Thr11)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 SH3BP1 SH3结构域结合蛋白3抗体
合格 合格 SH3BP2 SH3结构域结合蛋白2抗体
合格 phospho-SH3BP2 (Ser427) 磷酸化SH3结构域结合蛋白2抗体
合格 SH3BP5 SH3结构域结合蛋白5抗体
合格 SH3GL2/Endophilin A1 胞吞蛋白Endophilin 1抗体
合格 RBCK1/HBV associated factor 4 乙型肝炎X病毒相关蛋白4抗体
合格 RBM14 RNA结合蛋白14抗体
合格 RBM16 RNA结合蛋白16抗体
合格 RBM17 RNA结合蛋白17抗体
合格 合格 RBM22 RNA结合蛋白22抗体
合格 RBM25 RNA结合蛋白25抗体
合格 Matrilin 1 软骨基质蛋白抗体
合格 RBM34 RNA结合蛋白34抗体
合格 RBM35A/ESRP1 RNA结合蛋白35A抗体
合格 合格 RBM47 RNA结合蛋白47抗体
合格 RBMS1/Cervical cancer oncogene 4 宫颈癌癌基因4抗体
合格 RBPSUHL 转录因子RBPL抗体
合格 phospho-NFAT2 (Ser233) (Ser235 mouse) 磷酸化活化T细胞核因子1蛋白抗体
合格 phospho-NFkB p100/p52 (Ser870) 磷酸化细胞核因子p100基因结合核因子抗体
合格 Histone binding protein/SLBP 组蛋白结合蛋白SLBP抗体
合格 SLC10A6 溶质载体家族蛋白10成员A6抗体
合格 SLC1A4 溶质载体家族蛋白1成员A4抗体
合格 TMEM180 跨膜蛋白180抗体
合格 SLC22A2 溶质载体家族蛋白22成员A2抗体
合格 SLC22A7 溶质载体家族蛋白22成员A7抗体
合格 合格 SLC34A1 溶质载体家族蛋白34成员A1抗体
合格 SLC25A38 溶质载体家族蛋白25成员38抗体
合格 SLC26A3 溶质载体家族蛋白26成员3抗体
合格 SLC2A13 溶质载体家族蛋白2成员A13抗体
合格 SLC30A10 溶质载体家族蛋白30成员A10抗体
合格 SLC30A9 溶质载体家族蛋白30成员A9抗体
合格 SLC35D2 溶质载体家族蛋白35成员D2抗体
合格 合格 SLC38A1 溶质载体家族蛋白38成员1抗体
合格 SLC38A9 溶质载体家族蛋白38成员A8抗体