辫丑辞蝉辫丑辞-惭础搁碍2(罢丑谤596)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 6366R
英文名称辫丑辞蝉辫丑辞-惭础搁碍2(罢丑谤596)
中文名称磷酸化丝氨酸/苏氨酸蛋白激酶惭础搁碍2抗体
别 名ELKL motif kinase 1; MARK2(phospho T596); ELKL motif kinase; EMK-1; EMK1; MAP/microtubule affinity regulating kinase 2; MAP/microtubule affinity-regulating kinase 2; Mark2; MARK2_HUMAN; PAR1 homolog; Serine/threonine protein kinase MARK2; Serine/threonine-protein kinase MARK2.
说 明 书100ul
产物类型磷酸化抗体
研究领域信号转导 激酶和磷酸酶 细胞骨架
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
辫丑辞蝉辫丑辞-惭础搁碍2(罢丑谤596)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, Guinea Pig,
产物应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量88kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human MARK2 around the phosphorylation site of Thr596:SR(p-S)TF
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
辫丑辞蝉辫丑辞-惭础搁碍2(罢丑谤596)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009].
Function:
Serine/threonine-protein kinase involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4, MAPT/TAU, and RAB11FIP2. Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.
Subunit:
Homodimer. Interacts with PAK7/PAK5; leading to inhibit the protein kinase activity. Interacts (when phosphorylated at Thr-596) with YWHAZ. In case of infection, interacts with H.pylori CagA, leading to inhibit kinase activity and junctional and polarity defects.
Subcellular Location:
Cell membrane; Peripheral membrane protein. Cytoplasm. Note=Phosphorylation at Thr-596 by PRKCZ/aPKC and subsequent interaction with 14-3-3 protein YWHAZ promotes relocation from the cell membrane to the cytoplasm.
Tissue Specificity:
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
Post-translational modifications:
Autophosphorylated. Phosphorylated at Thr-208 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-208 by TAOK1 activates the kinase activity, leading to phosphorylation and detachment of MAPT/TAU from microtubules. Phosphorylation at Ser-212 by GSK3-beta (GSK3B) inhibits the kinase activity. Phosphorylation by CaMK1 promotes activity and is required to promote neurite outgrowth. Phosphorylation at Thr-596 by PRKCZ/aPKC in polarized epithelial cells inhibits the kinase activity and promotes binding to 14-3-3 protein YWHAZ, leading to relocation from cell membrane to cytoplasm.
Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain.
Gene ID:
2011
辫丑辞蝉辫丑辞-惭础搁碍2(罢丑谤596)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
辫丑辞蝉辫丑辞-惭础搁碍2(罢丑谤596)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 phospho-MST4 + MST3 + STK25 (Thr178 + Thr190 + Thr174) 磷酸化丝氨酸/苏氨酸蛋白激酶MST4,MST3,STK25抗体
合格 合格 Caspase-1 p20 天冬氨酸-胱氨酸特异性蛋白酶家族抗体
合格 STOM 红细胞膜蛋白STOM抗体
合格 NSE 神经元特异性烯醇化酶/γ 烯醇化酶抗体
合格 HPV16 E7 人类**状瘤病毒16-E7抗体
合格 合格 phospho-HSP70 (Tyr611) 磷酸化热休克蛋白-70抗体
合格 phospho-HSP70 (Tyr41) 磷酸化热休克蛋白-70抗体
合格 Phospho-CD18 (Ser756) 磷酸化整合素β2/Integrin β2抗体
合格 合格 Phospho-CD18 (Thr758) 磷酸化整合素β2/Integrin β2抗体
合格 合格 ADRA1B alpha 1肾上腺素能受体B抗体
合格 DRAK2 DAP凋亡诱导蛋白激酶2抗体
合格 CCL4 巨噬细胞炎症因子1β /MIP-1β抗体
合格 DYNC1I1 胞浆动力蛋白中间链1抗体
合格 DYNC1I2 胞浆动力蛋白中间链2抗体
合格 合格 CATSPER2 阳离子通道精子相关蛋白2抗体
合格 合格 Serine racemase 丝氨酸消旋酶抗体
合格 Complement C5 beta chain 补体C5β链抗体
合格 MyD88 髓样分化蛋白抗体
合格 合格 Heparanase 乙酰肝素酶抗体
合格 Podoplanin 平足蛋白/**管内皮细胞蛋白抗体
合格 phospho-KMT6 (Ser21) 磷酸化抑癌蛋白EZH2抗体
合格 CD3 CD3抗体
合格 CHRNA7 烟碱型乙酰胆碱受体α7抗体