Phospho-NDRG1 (Thr346)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 3298R
英文名称Phospho-NDRG1 (Thr346)
中文名称磷酸化分化相关基因狈顿搁骋1抗体
别 名NDRG1 (Phospho Thr346); NDRG1 (Phospho T346); p-NDRG1 (Thr346); N-myc downstream regulated gene 1; TDD5; 42 kDa; cap43; cmt4d; Differentiation related gene1 protein; Drg 1; drg1; gc4; hmsnl; Human mRNA for RTP complete cds; N myc downstream regulated gene 1 protein; Ndr 1; NDRG 1; Nickel specific induction protein Cap43; Nmyc downstream regulated gene1; Protein NDRG1; Protein regulated by oxygen 1 ; Protein regulated by oxygen1; proxy1; reducin; Reducing agents and tunicamycin responsive protein; rit42; rtp; targ1; tdds; tunicamycin-responsive protein.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 细胞生物 **学 神经生物学 信号转导 转录调节因子
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
Phospho-NDRG1 (Thr346)抗体交叉反应 Human, Mouse, Rat, Dog, Cow, Horse,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量43kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human NDRG1 around the phosphorylation site of Thr346:SH(p-T)SE
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-NDRG1 (Thr346)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Function:
Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.
Subunit:
Interacts with RAB4A (membrane-bound form); the interaction involves NDRG1 in vesicular recycling of CDH1.
Subcellular Location:
Cytoplasm, cytosol. Cytoplasm, cytoskeleton, centrosome. Nucleus. Cell membrane. Note=Mainly cytoplasmic but differentially localized to other regions. Associates with the plasma membrane in intestinal epithelia and lactating mammary gland. Translocated to the nucleus in a p53/TP53-dependent manner. In prostate epithelium and placental chorion, located in both the cytoplasm and in the nucleus. No nuclear localization in colon epithelium cells. In intestinal mucosa, prostate and renal cortex, located predominantly adjacent to adherens junctions. Cytoplasmic with granular staining in proximal tubular cells of the kidney and salivary gland ducts. Recruits to the membrane of recycling/sorting and late endosomes via binding to phosphatidylinositol 4-phosphate. Associates with microtubules. Colocalizes with TUBG1 in the centrosome. Cytoplasmic location increased with hypoxia. Phosphorylated form found associated with centromeres during S-phase of mitosis and with the plasma membrane.
Tissue Specificity:
Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Also expressed in heart, brain, skeletal muscle, lung, liver and pancreas. Low levels in peripheral blood leukocytes and in tissues of the immune system. Expressed mainly in epithelial cells. Also found in Schwann cells of peripheral neurons. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
Post-translational modifications:
Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent.
DISEASE:
Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
Similarity:
Belongs to the NDRG family.
SWISS:
Q92597
Gene ID:
10397
Phospho-NDRG1 (Thr346)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-NDRG1 (Thr346)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 FAM161B FAM161B蛋白抗体
合格 FAM164B FAM164B蛋白抗体
合格 FAM101A FAM101A蛋白抗体
合格 FRMD1 FRMD1蛋白抗体
合格 CTDSPL2 CTDSPL2蛋白抗体
合格 DEPTOR DEPTOR蛋白抗体
合格 TBX1 先心病相关蛋白TBX1抗体
合格 DHRS13 短链脱氢还原酶13抗体
合格 DHRS7 视网膜短链脱氢酶/还原酶家族7抗体
合格 DHRS4 短链脱氢酶/还原酶家族4抗体
合格 DHRS4L2 短链脱氢酶/还原酶家族4样2蛋白抗体
合格 GIMAP1 GTP酶IMAP家族成员1抗体
合格 GIMAP2 GTP酶IMAP家族成员2抗体
合格 GIMAP6 GTP酶IMAP家族成员6抗体
合格 合格 DQX1 DQX1蛋白抗体
合格 合格 TXNRD1 硫氧环蛋白还原酶1抗体
合格 Hi95 缺氧诱导基因95抗体
合格 SRXN1 抗氧化蛋白1抗体
合格 TMEM173 跨膜蛋白173抗体
合格 UBE2D2 泛素蛋白连接酶D2抗体
合格 UBE2E2 泛素蛋白连接酶2E2抗体
合格 UBE2D4 泛素蛋白连接酶D4抗体
合格 phospho-NIPA(Ser354) 磷酸化间变性**瘤激酶核相互作用伴侣蛋白抗体
合格 UBE2O 泛素结合酶E2 230K抗体
合格 UBE2Q1 泛素蛋白连接酶Q1抗体