phospho-NFAT2 (Ser233) (Ser235 mouse)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 19785R
英文名称phospho-NFAT2 (Ser233) (Ser235 mouse)
中文名称磷酸化活化罢细胞核因子1蛋白抗体
别 名NFAT2 (phospho S233); p-NFAT2 (phospho S233); NFAT2; MGC138448; NF ATc; NF ATc1; NFAT 2; NFATc1; NFAT transcription complex cytosolic component; NFATC 1; NFATc; NFATc1; Nuclear factor of activated T cells cytoplasmic 1; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1; Nuclear factor of activated T cells cytosolic component 1; NFAC1_HUMAN.
说 明 书100ul
产物类型磷酸化抗体
研究领域细胞生物 神经生物学 细胞凋亡 转录调节因子 t-**细胞 表观遗传学
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
phospho-NFAT2 (Ser233) (Ser235 mouse)抗体交叉反应 Human, Mouse, Rat, Pig, Cow, Sheep,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量101kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human NFAT2 around the phosphorylation site of Ser233:LG(p-S)SP
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
phospho-NFAT2 (Ser233) (Ser235 mouse)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability. The biological function of this helicase has not yet been determined. Two alternatively spliced transcripts, which encode the same isoform but differ in their 5' and 3' UTRs, have been described. [provided by RefSeq, Jul 2008]
Function:
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
Subcellular Location:
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Tissue Specificity:
Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.
Post-translational modifications:
Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
Similarity:
Contains 1 RHD (Rel-like) domain.
SWISS:
O95644
Gene ID:
4772
phospho-NFAT2 (Ser233) (Ser235 mouse)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
phospho-NFAT2 (Ser233) (Ser235 mouse)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 CCDC54 卷曲螺旋结构域蛋白54抗体
合格 合格 CCDC83 卷曲螺旋结构域蛋白83抗体
合格 CCDC87 卷曲螺旋结构域蛋白87抗体
合格 FAM36A FAM36A蛋白抗体
合格 FAM134C FAM134C蛋白抗体
合格 CHD3 ATP依赖的解旋酶CHD3抗体
合格 RB1CC1/FIP200 RB1的诱导卷曲蛋白RB1CC1抗体
合格 CHCHD6 卷曲螺旋结构域蛋白CHCHD6抗体
合格 PHF8 组蛋白赖氨酸去甲基化酶PHF8抗体
合格 KIF11 驱动蛋白样蛋白1抗体（甲状腺**受体相互作用蛋白5）
合格 合格 COMMD9 铜代谢结构域蛋白9抗体
合格 合格 COMMD8 铜代谢结构域蛋白8抗体
合格 COMMD3 铜代谢结构域蛋白3抗体
合格 AVSD2 富含半胱氨酸与表皮生长因子样蛋白2抗体
合格 合格 CRELD2 富含半胱氨酸与表皮生长因子样蛋白2抗体
合格 合格 BRN4 脑转录因子4蛋白抗体
合格 Nestin 巢蛋白/神经上皮干细胞蛋白抗体
合格 合格 FAM113A FAM113A蛋白抗体
合格 FAM13C1 FAM13C1蛋白抗体
合格 FAM59A FAM59A蛋白抗体
合格 FAM151A FAM151A蛋白抗体
合格 FAM153B FAM153B蛋白抗体
合格 FAM154A FAM154A蛋白抗体
合格 FAM155A FAM155A蛋白抗体
合格 FAM160B1 FAM160B1蛋白抗体