Phospho-PAK4(Ser474) + PAK5(Ser602) + PAK6(Ser560)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 3314R
英文名称Phospho-PAK4(Ser474) + PAK5(Ser602) + PAK6(Ser560)
中文名称磷酸化辫21激活激酶4/5/6抗体
别 名PAK4 (phospho S474); p-PAK4 (phospho S474); Phospho-PAK4(Ser474); Phospho-PAK5(Ser602); Phospho-PAK6(Ser560); PAK4 + PAK5 + PAK6 (phospho S474 + S602 + S560); p21 activated kinase 4 ; p21(CDKN1A) activated kinase 4; PAK 4; Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs; Serine threonine kinase PAK 4; Serine/threonine protein kinase PAK 4; Serine/threonine protein kinase PAK4; KIAA1142; PAK4_HUMAN.
JournalPMIDIFApplicationJournal of Bone and Mineral Research (2015)256406986.5890WB
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 **学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
Phospho-PAK4(Ser474) + PAK5(Ser602) + PAK6(Ser560)抗体交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
产物应用WB=1:500-2000 ELISA=1:500-1000 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量64kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human PAK4 around the phosphorylation site of PAK4 Ser474 [RK(p-S)LV]:RK(p-S)LV
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-PAK4(Ser474) + PAK5(Ser602) + PAK6(Ser560)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes, including the cell cycle, dynamics of the cytoskeleton, apoptosis, oncogenic transformation, and transcription. All PAK family members are characterized by the presence of p21-binding domain. p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, and lipids, which stimulate autophosphorylation and phosphorylation of exogenous substrates. Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo. Phosphospecific antibodies directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42. Current data strongly implicates PAK-4 in oncogenesis. PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins.
Function:
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Subunit:
Interacts with FGFR2 and GRB2. Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1.
Subcellular Location:
Cytoplasm. Note=Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways.
Tissue Specificity:
Highest expression in prostate, testis and colon.
Post-translational modifications:
Autophosphorylated on serine residues when activated by CDC42/p21.
Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity).
Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Gene ID:
10298
Phospho-PAK4(Ser474) + PAK5(Ser602) + PAK6(Ser560)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-PAK4(Ser474) + PAK5(Ser602) + PAK6(Ser560)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 Phospho-Zyxin (Ser142+Ser143) 磷酸化斑联蛋白抗体
合格 Delta Opioid Receptor D型受体抗体
合格 PKC delta 蛋白激酶C亚性D型抗体
合格 合格 Synaptopodin 突触足蛋白抗体
合格 RACK1 受体激活蛋白激酶C1抗体
合格 HOXB4 HOXB4抗体
合格 FPRL1 脂氧素受体1抗体
合格 KCNA7 钾电压阀门通道混合器相关亚家族成员7抗体
合格 FLASH 半胱氨酸蛋白酶8相关蛋白2抗体
合格 NIPP1 核抑制蛋白磷酸酶1抗体
合格 Caspase-7 p20 半胱胺酸蛋白酶蛋白-7抗体
合格 Calmodulin 钙调节素/钙调蛋白/钙调素抗体
合格 SLC44A1 溶质转运蛋白家族44成员1抗体
合格 Brs3 蛙皮素受体3抗体
合格 BMP6 骨形态发生蛋白6抗体
合格 HSP22 热休克蛋白-22抗体
合格 PBR 外周苯二氮受体抗体
合格 RALDH2 视黄醛脱氢酶2型抗体
合格 COMP 软骨寡聚基质蛋白抗体
合格 Nox1 有丝分裂氧化酶1抗体
合格 合格 CD1d T**细胞CD1D抗体
合格 REG4 再生基因蛋白4抗体
合格 Neurogenin 2 神经元素2抗体
合格 合格 AF10 髓系、**、混合系白血病易位基因10抗体
合格 Phospho-p70 S6 Kinase Beta (Thr444 + Ser447) 磷酸化核糖体S6蛋白激酶β2抗体