Phospho-Pin1 (Ser16)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 3354R
英文名称Phospho-Pin1 (Ser16)
中文名称磷酸化肽基脯氨酞顺反异构酶笔颈苍濒抗体
别 名DOD; NIMA interacting 1; Peptidyl prolyl cis trans isomerase NIMA interacting 1; Peptidyl prolyl cis/trans isomerase NIMA interacting; Pin 1; PPIase Pin1; Prolyl isomerase; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1; Protein NIMA interacting 1; Rotamase Pin1; UBL 5; UBL5; PIN1_HUMAN.
说 明 书100ul
产物类型磷酸化抗体
研究领域肿瘤 **学 神经生物学 信号转导 干细胞 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
Phospho-Pin1 (Ser16)抗体交叉反应 Human, Mouse, Rat, Dog, Cow,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量18kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human Pin1 around the phosphorylation site of Ser16:RM(p-S)RS
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Phospho-Pin1 (Ser16)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
Pin1 is a Peptidyl-prolyl isomerases (PPIase). Peptidyl-prolyl isomerases (PPIase) facilitate the cis-trans interconversion of the peptidyl-prolyl bond thereby affecting protein folding. Pin1 is a PPIase which specifically recognizes phosphorylated S/T-P bonds. Pin1 has been implicated in tau pathologies that underlie Alzheimer's Disease. Pin1 binds to tau phosphorylated specifically on the Thr231-Pro site and induces conformational changes in tau. Such conformational changes can directly restore the ability of phosphorylated Tau to bind microtubules and promote microtubule assembly and/or facilitate tau dephosphorylation. Pin1 expression inversely correlates with the predicted neuronal vulnerability in normally aged brain and also with actual neurofibrillary degeneration in AD brain. Pin1 could be pivotal for maintainance of normal neuronal function and preventing age-dependent neurodegeneration.
Function:
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation.
Subunit:
Interacts with STIL (By similarity). Interacts with KIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX. Interacts with BTK. Interacts (via PpiC domain) with DAPK1. Interacts with the phosphorylated form of RAF1.
Subcellular Location:
Nucleus. Nucleus speckle. Cytoplasm.
Tissue Specificity:
The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Ser-71 by DAPK1 results in inhibition of its catalytic activity, nuclear localization, and its ability to induce centrosome amplification, chromosome instability and cell transformation.
Similarity:
Contains 1 PpiC domain.
Contains 1 WW domain.
SWISS:
Q13526
Gene ID:
5300
Phospho-Pin1 (Ser16)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
Phospho-Pin1 (Ser16)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
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合格 合格 IL-13Ra2 白细胞介素13受体a2抗体
合格 合格 PERK 蛋白激酶样内质网激酶抗体
合格 ICOS ligand 诱导协同刺激分子配体CD275抗体
合格 CD93 CD93抗体
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合格 MIP3/CCL23 巨噬细胞炎性蛋白抗体
合格 合格 phospho-JAK2(Tyr1007+Tyr1008) 磷酸化蛋白酪氨酸激酶JAK-2抗体
合格 SLAMF9 CD2F-10抗体
合格 CD5L CD5L抗体
合格 CD9 CD9蛋白抗体
合格 合格 CD23 CD23/FcεRII抗体
合格 合格 合格 CD30 CD30抗体
合格 CD43 CD43抗体
合格 CD48 CD48抗体
合格 CD58/LFA3 **细胞功能相关抗原-3抗体
合格 CD69 活化诱导分子CD69抗体
合格 eEF1A1 翻译延伸因子EF-1a抗体
合格 CD44 CD44抗体
合格 合格 CD11c 整合素αX抗体
合格 AIMP2 AIMP2抗体
合格 合格 CCR5 细胞表面趋化因子受体5抗体
合格 CD72 CD72蛋白抗体