辫丑辞蝉辫丑辞-搁翱颁碍1(罢丑谤455+厂别谤456)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 10239R
英文名称辫丑辞蝉辫丑辞-搁翱颁碍1(罢丑谤455+厂别谤456)
中文名称磷酸化搁丑辞相关蛋白激酶1抗体
别 名p-ROCK1(Thr455/Ser456); p160 ROCK1; p160ROCK; Renal carcinoma antigen NY REN 35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho-associated coiled-coil containing protein kinase 1; ROCK1_HUMAN.
说 明 书100ul
产物类型磷酸化抗体
研究领域信号转导 激酶和磷酸酶
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
辫丑辞蝉辫丑辞-搁翱颁碍1(罢丑谤455+厂别谤456)抗体交叉反应 Human, Mouse, Rat, Pig, Cow, Horse,
产物应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量158kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human ROCK1 around the phosphorylation site of Thr455+Ser456:CR(p-T)(p-S)NI
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
辫丑辞蝉辫丑辞-搁翱颁碍1(罢丑谤455+厂别谤456)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
Function:
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Subunit:
Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not.
Subcellular Location:
Cytoplasm. Note=The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
Post-translational modifications:
Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
DISEASE:
Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
Similarity:
Belongs to the Cu-Zn superoxide dismutase family.
SWISS:
Q13464
Gene ID:
6093
辫丑辞蝉辫丑辞-搁翱颁碍1(罢丑谤455+厂别谤456)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
辫丑辞蝉辫丑辞-搁翱颁碍1(罢丑谤455+厂别谤456)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 LYZL2 溶菌酶样蛋白2抗体
合格 LZTR2 亮氨酸拉链样转录调节因子1抗体
合格 LZTS2 亮氨酸拉链抑癌蛋白2抗体
合格 SARDH 肌氨酸脱氢酶抗体
合格 CRLR 降钙素基因相关肽1型受体抗体
合格 MAFF 转录因子MAFF抗体
合格 MAGEA6 黑色素瘤相关抗原6抗体
合格 MAGEB10 黑色素瘤相关抗原B10抗体
合格 MAGED2 黑色素瘤相关抗原D2抗体
合格 MAGIX MAGIX蛋白抗体
合格 MAGT1 镁转运蛋白1抗体
合格 MAK16/RBM13 RNA结合蛋白MAK16抗体
合格 MAK3/C14orf35 14号染色体开放阅框35抗体
合格 MAMSTR MAMSTR蛋白抗体
合格 MAN2A2 α-甘露糖苷酶2x抗体
合格 MANBA β-甘露糖苷酶抗体
合格 MANEL MANEL蛋白抗体
合格 MAP1D 蛋氨酸氨肽酶1D抗体
合格 合格 Parkin 帕金蛋白抗体
合格 MAP1S 微管相关蛋白1S抗体
合格 合格 MAP3K13/LZK MAP3K13蛋白抗体
合格 MAPKAP3 丝裂原活化蛋白激酶活化的蛋白激酶3抗体
合格 MAPKAPK2/3 丝裂原活化蛋白激酶活化的蛋白激酶2/3抗体
合格 PD-1 程序性死亡1抗体
合格 MARS 甲硫氨酸转运RNA合成酶抗体
合格 MARS2 甲硫氨酸转运RNA合成酶2抗体
合格 MARVELD3 MARVEL蛋白家族D3抗体
合格 MBOAT1 溶血磷脂酰酰基转移酶1抗体
合格 phospho-MCAK (Ser95) 磷酸化有丝分裂着丝粒相关驱动蛋白抗体