辫丑辞蝉辫丑辞-搁翱颁碍2(罢测谤256)抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 5676R
英文名称辫丑辞蝉辫丑辞-搁翱颁碍2(罢测谤256)
中文名称磷酸化搁丑辞相关蛋白激酶2抗体
别 名ROCK2(phospho Y256); p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; ROCK2_HUMAN.
说 明 书100ul
产物类型磷酸化抗体
研究领域**学 信号转导 转录调节因子
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
辫丑辞蝉辫丑辞-搁翱颁碍2(罢测谤256)抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Guinea Pig,
产物应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量153kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ROCK2 around the phosphorylation site of Tyr256:PD(p-Y)IS
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
辫丑辞蝉辫丑辞-搁翱颁碍2(罢测谤256)抗体保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产物介绍产补肠办驳谤辞耻苍诲:
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Function:
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus.
Subunit:
Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1.
Subcellular Location:
Cytoplasm. Cell membrane; Peripheral membrane protein (By similarity). Nucleus. Cytoplasm, cytoskeleton, centrosome.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Tyr-722 reduces its binding to RHOA and is crucial for focal adhesion dynamics. Dephosphorylation by PTPN11 stimulates its RHOA binding activity.
Cleaved by granzyme B during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.
SWISS:
O75116
Gene ID:
9475
辫丑辞蝉辫丑辞-搁翱颁碍2(罢测谤256)抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
辫丑辞蝉辫丑辞-搁翱颁碍2(罢测谤256)抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 ZDHHC6 ZDHHC6蛋白抗体
合格 ZDHHC8/ZNF378 锌指蛋白378抗体
合格 alpha MSH 促黑细胞**α抗体
合格 Midkine 中期因子/肝素结合生长因子抗体
合格 ZNF289/ARFGAP2 锌指蛋白289抗体
合格 ZNF24 锌指蛋白24抗体
合格 合格 ZNF365 锌指蛋白365抗体
合格 NR3C2/Mineralocorticoid receptor 盐皮质**受体抗体
合格 ZNF699 锌指蛋白699抗体
合格 ZNF642 锌指蛋白642抗体
合格 ZFAND2B 锌指蛋白AN1-2B抗体
合格 ZFP14 锌指蛋白14抗体
合格 ZFP161 锌指蛋白161抗体
合格 ZC4H2 ZC4H2蛋白抗体
合格 PIP5K2B 磷脂酰肌醇-5-磷酸盐-4激酶2型β抗体
合格 CLEC16A C型凝集素结构域家族16成员A抗体
合格 CCK4/PTK7 蛋白酪氨酸激酶7抗体
合格 MACF1/ACF7 肌动蛋白交联蛋白家族7抗体
合格 ZCCHC18 ZCCHC18蛋白抗体
合格 MMP12 基质金属蛋白酶-12抗体
合格 ZCCHC4 ZCCHC4蛋白抗体
合格 ZCCHC5 ZCCHC5蛋白抗体
合格 ZCCHC6 ZCCHC6蛋白抗体
合格 ZCCHC7 ZCCHC7蛋白抗体
合格 ZCCHC16 ZCCHC16蛋白抗体
合格 MAB21L1 MAB21L1蛋白抗体
合格 ZCCHC9 ZCCHC9蛋白抗体
合格 ZCPW1 ZCPW1蛋白抗体
合格 Netrin 1 轴突导向因子1抗体