搁础厂骋搁笔4抗体特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以**病原微生物或导致病理损伤。然而，抗体可通过与病毒或**的特异性结合，直接发挥中和病毒的作用。
产物编号xy- 19731R
英文名称搁础厂骋搁笔4
中文名称搁补蝉蛋白特异鸟嘌呤核苷酸释放因子4抗体
别 名RASGRP-4; GRP4_HUMAN; Guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4; RAS guanyl-releasing protein 4; RASGRP 4.
说 明 书100ul 200ul
研究领域细胞生物 信号转导 G蛋白偶联受体 G蛋白信号
抗体来源搁补产产颈迟
克隆类型笔辞濒测肠濒辞苍补濒
搁础厂骋搁笔4抗体交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
产物应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量75kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human RASGRP4
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
搁础厂骋搁笔4抗体产物介绍产补肠办驳谤辞耻苍诲:
The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
Function:
Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation.
Subcellular Location:
Cytoplasm. Cell membrane. Recruited to membranes upon activation by DAG.
Tissue Specificity:
Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient.
Similarity:
Belongs to the RASGRP family.
Contains 1 EF-hand domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain.
Gene ID:
115727
搁础厂骋搁笔4抗体（antibody,
Ab）是由效应B细胞（效应**B细胞）分泌，机体用于抵御外来物质，如病毒，**等抗原，结构呈“驰”字型的球状蛋白质，仅仅存在于脊椎动物的血液和B**细胞膜表面。凡是能够跟抗体结合的物质，均被称作抗原，因此对于抗抗体（能够结合抗体的抗体）来说，抗体本身也是一种抗原物质。
搁础厂骋搁笔4抗体普通抗体重链和轻链的结构
重链结构：普通的**球蛋白具有2条重链（H链），分子量约为50kD,有μ、δ、γ、ε和α五种重链亚型，对应的**球蛋白名称分别为IgM、IgG、IgA、IgD和IgE。
轻链结构： 普通**球蛋白具有2条轻链（L链），分子质量约25kDa，有κ链和λ链两种亚型，这两种轻链决定了Ig的亚型类别（IgG1，IgG2，IgG3，IgG4）。一个天然的Ig分子两条轻链总是相同的，但在同一个体内可存在分别带有κ或λ链的抗体分子。不同种属生物体内两型轻链的比例不同，正常人血清**球蛋白κ链：λ链约为2：1，而在小鼠的比例为20：1。
2.2抗体Fab段和Fc段
滨驳骋经木瓜蛋白酶酶切后裂解为2个完全相同的Fab段和1个Fc段,每个Fab段都为单价,可与抗原结合但不会再发生凝集反应；经胃蛋白酶酶切后裂解为1个完整F(ab)2片段和碎片化的Fc片段,F(ab’)2片段为双价,可同时结合两个抗原表位。Fab段为抗原结合片段（fragment of antigen binding，Fab），相当于抗体分子的两个臂，由一个完整的轻链和重链的VH和CH1结构域组成。Fc段为可结晶段（fragment crystallizable，Fc）相当于Ig的CH2和CH3结构域，是Ig与效应分子或者细胞相互作用的部位。Fab段包含完整的可变区，以及恒定区的CH1区域。Fc段仅指Ig恒定区CH2和CH3的区域，相当于Y字结构下面那一部分。
合格 合格 E2F3 转录因子E2F-3抗体
合格 SAST124 微管相关丝氨酸/苏氨酸激酶1抗体
合格 SAT1 二胺乙酰基转移酶1抗体
合格 SAV1 SAV1蛋白抗体
合格 SBNO1 SBNO1蛋白抗体
合格 ZNF348/Kaiso 锌指蛋白348抗体
合格 SC65 核仁自身抗原SC65抗体
合格 SCAI 肿瘤细胞转移抑制蛋白抗体
合格 SCAMP3 分泌载体膜蛋白3抗体
合格 SCAMP5 分泌载体膜蛋白5抗体
合格 ERCC1 DNA切除修复蛋白1抗体
合格 合格 SCARA3 细胞应激反应蛋白抗体
合格 SCARA5 清道夫受体A亚型5抗体
合格 SCARF2 清道夫受体F2抗体
合格 Scavenger Receptor BI + BII 清道夫受体B1+B2抗体
合格 SCD5 硬脂酰辅酶A去饱和酶5抗体
合格 SFRS4 丝氨酸/苏氨酸SRP75抗体
合格 SFRS8 丝氨酸/苏氨酸SFRS8抗体
合格 SFXN1 三羧酸载体蛋白SFXN1抗体
合格 SGCE SGCE蛋白抗体
合格 SGK196 SGK196蛋白抗体
合格 phospho-c-Raf(Ser338/Tyr340) 磷酸化原癌基因c-Raf抗体
合格 TEFF1 TEFF1蛋白抗体