础罢笔依赖解旋酶搁贰狈罢1抗体
规格：1尘驳/1尘濒
英文名： RENT1+hUPF1
别名： ATP dependent helicase RENT1; ATP-dependent helicase RENT1; Delta helicase; HUPF 1; HUPF1; KIAA0221; Nonsense mRNA reducing factor 1; NORF 1; NORF1; pNORF 1; pNORF1; Regulator of nonsense transcripts
分子量： 124kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human RENT1/hU
交叉反应：Human, Mouse, Rat, Chicken, Dog, Cow, Horse,
细胞定位：细胞浆
础罢笔依赖解旋酶搁贰狈罢1抗体产物介绍：background: In eukaryotes, it is essential to have the ability to detect and degrade transcripts that lack full coding potential. Nonsense-mediated RNA decay (NMD) protects the organism by avoiding the translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability (1–3). Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Function: Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic础罢笔依赖解旋酶搁贰狈罢1抗体 viability. Subunit: Found in a post-splicing messenger ribonucleoprotein (mRNP) complex. Associates with the exon junction complex (EJC). Associates with the SGM1C complex; is phosphorylated by the complex kinase component SGM1. Interacts with UPF2, UPF3A and UPF3B. Interacts with EST1A and SLBP. Interacts (when hyperphosphorylated) with PNRC2. Interacts with EIF2C1, EIF2C2 and GSPT2. Subcellular Location: Cytoplasm. Cytoplasm, P-body. Note=Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated by SMG1; required for 础罢笔依赖解旋酶搁贰狈罢1抗体formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the DNA2/NAM7 helicase family. Contains 1 C2H2-type zinc finger. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
础罢笔依赖解旋酶搁贰狈罢1抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid