细胞周期调控因子14础抗体
规格：1尘驳/1尘濒
英文名： Cdc14A
别名： CDC 10; Cdc 14; Cdc 14A; Cdc 14A1; Cdc 14A2; CDC10; Cdc14; CDC14 cell division cycle 14 homolog A; CDC14 homolog A; Cdc14A1; Cdc14A2; Cell division cycle 14 homolog A; Dual specificity protein phospha
分子量： 67kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human Cdc14A
交叉反应：Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
细胞定位：细胞核 细胞浆
细胞周期调控因子14础抗体产物介绍：background: In budding yeast, the Cdc14a phosphatase activates mitotic exit by dephosphorylation of specific cyclin-dependent kinase (Cdk) substrates and seems to be regulated by sequestration in the nucleolus until its release during mitosis. Human Cdc14a phosphatase is highly similar to Saccharomyces cerevisiae Cdc14 and is a member of the dual specificity protein Tyrosine phosphatase family. It interacts with and dephosphorylates tumor suppressor protein p53 and may regulate the function of p53. In addition, Cdc14a dephosphorylates hCdh1 and activates APCCdh1. Cdc14a phosphatase plays a role in the regulation of the centrosome cycle, mitosis and cytokinesis, thereby influencing chromosome partitioning and genomic stability in human cells. Deregulated human Cdc14a phosphatase disrupts centrosome separation and chromosome segregation. Function: Overproduction of hCdc14A leads to progressive cell death, accompanied by accumulation of pre-G1 DNA fragments and gradual elimination of cells at the G2/M transition. In 50% of the mitotic cells, multipolar mitotic spindles and misaggregated chromosomes are found. hCdc14A localizes to the centrosomes at the cell-cycle interphase stage. When the cell enters mitosis, this localization细胞周期调控因子14础抗体 disappears suggesting that hCdc14A dissociates from the centrosomes at the G2/M transition. hCdc14A localizes to interphase centrosomes, but not to mitotic centrosomes, while hCdc14B localizes to the interphase nucleolus. Thus each isoform regulates separate cell cycle events. Cdc14A may affect cell cycle progression by its ability to dephosphorylate p53 at Ser315. This phosphatase activity is mediated by the interaction between the N-terminus of hCdc14A and the C-terminus of p53. Subunit: Interacts with KIF20A, which is required to localize CDC14 to the 细胞周期调控因子14础抗体midzone of the mitotic spindle. Subcellular Location: Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Centrosomal during interphase, released into the cytoplasm at the onset of mitosis. Subsequently localizes to the midzone of the mitotic spindle. Tissue Specificity: Widely expressed. Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. Database links: UniProtKB/Swiss-Prot: Q9UNH5.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产细胞周期调控因子14础抗体品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 细胞周期蛋白 激酶和磷酸酶 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid