厂贰惭颁础笔3蛋白抗体
规格：1尘驳/1尘濒
英文名： SEMCAP3
别名： Ligand of Numb protein X 3; likely ortholog of mouse semaF cytoplasmic domain associated protein 3; LNX3; PDZ domain containing ring finger 3; Protein SEMACAP3; SEMACAP3; Semaphorin cytoplasmic domain
分子量： 120kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human SEMCAP3/
交叉反应：Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep,
细胞定位：
厂贰惭颁础笔3蛋白抗体产物介绍：background: PDZRN3 contains a RING-finger motif in its N-terminal region, two PDZ domains in its central region and a consensus-binding motif for PDZ domains at its C-terminus. It was identified in silico as a homolog of the protein known as LNX1 or SEMCAP1, which possesses ubiquitin ligase activity and binds the membrane protein Semaphorin 4C. However, PDZRN3 itself has not previously been characterized. We have now evaluated the properties and functions of PDZRN3. The PDZRN3 gene was shown to be expressed in various human tissues including the heart, skeletal muscle and liver and its expression in mouse skeletal muscle was developmentally regulated. Both the differentiation of C2C12 mouse skeletal myoblasts into myotubes and injury-induced muscle regeneration in vivo were found to be accompanied by up-regulation of PDZRN3. The differentiation-associated increase in the expression of PDZRN3 in C2C12 cells follows that of myogenin 厂贰惭颁础笔3蛋白抗体and precedes that of myosin heavy chain. Depletion of PDZRN3 by RNA interference inhibited the formation of myotubes as well as the associated up-regulation of myosin heavy chain in C2C12 cells. Our data suggest that PDZRN3 plays an essential role in the differentiation of myoblasts into myotubes by acting either downstream or independently of myogenin. Function: E3 ubiquitin-protein ligase. Plays an important role in regulating the surface level of MUSK on myotubes. Mediates the ubiquitination of MUSK, promoting its endocytosis and lysosomal degradation. Might contribute to terminal myogenic differentiation (By similarity). Subunit: Interacts with NLGN1 and EFNB2 (By similarity). Interacts with UBE2D2 and with MUSK via the first PDZ domain (By similarity). Tissue Specificity: SEMCAP3 is widely expressed at high levels, and is thought to be involved in protein/zinc ion binding. Post-translational modifications: Auto-ubiquitinated (By similarity). Similarity: Contains 2 PDZ (DHR) domains. Contains 1 RING-type zinc finger. 厂贰惭颁础笔3蛋白抗体Contains 1 TRAF-type zinc finger. Gene ID: 23024 Database links: Entrez Gene: 23024 Human Entrez Gene: 55983 Mouse Entrez Gene: 312607 Rat Omim: 609729 Human SwissProt: Q9UPQ7 Human SwissProt: Q69ZS0 Mouse SwissProt: P68907 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
厂贰惭颁础笔3蛋白抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 发育生物学 细胞周期蛋白 细胞分化
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid