贰4贵转录因子1抗体
规格：1尘驳/1尘濒
英文名： E4F1
别名： E4F; E4F transcription factor 1, isoform CRA_b; P120E4F transcription factor; E4F1_HUMAN.
分子量： 83kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human E4F1
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
细胞定位：细胞核 细胞浆
贰4贵转录因子1抗体产物介绍：background: E4F1, also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains nine C2H2-type zinc fingers. Expressed ubiquitously in and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control. Additionally, E4F1 is thought to function as a ubiquitin ligase that mediates the ubiquitination (and subsequent degradation) of target proteins and may be involved in the p53 tumor suppressor pathway. E4F1, which may be post-translationally phosphorylated or sumoylated, is subject to proteolytic cleavage which results in the creation of a short peptide with specific DNA binding capabilities. Function: May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription. [FUNCTION] Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and Subunit: Homodimer; binds DNA as a dimer. Forms a complex with CDKN2A and TP53. Interactions with TP53, RB1, ANP32A, BMI1 and FHL2 regulate E4F1 activity. Interacts with HDAC1, 贰4贵转录因子1抗体HMGA2 and RASSF1. Interacts with HBV protein X. Subcellular Location: Nucleus, nucleoplasm. Cytoplasm. Note=A small fraction is detected in the cytoplasm. Excluded from the nucleolus where it is targeted upon CDKN2A overexpression. Localizes to the mitotic spindle during embryogenesis (By similarity). Tissue Specificity: Ubiquitously expressed. Post-translational modifications: Proteolytic cleavage produces a 50 kDa N-terminal peptide (p50E4F) which has a DNA-binding activity and activates transcription in presence of the adenoviral E1A protein. The major full length protein (p120E4F) functions as a repressor of transcription. Phosphorylated; p120E4F and p50E4F are both phosphorylated. Phosphorylation is cell 贰4贵转录因子1抗体cycle-dependent and differentially regulates DNA-binding activity and function of both forms. May be sumoylated by UBE2I upon interaction with CDKN2A. Similarity: Contains 9 C2H2-type zinc fingers. Gene ID: 1877 Database links: Entrez Gene: 1877 Human Omim: 603022 Human SwissProt: Q66K89 Human Unigene: 513268 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
贰4贵转录因子1抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学 信号转导 转录调节因子 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid