嘌呤嘧啶核酸内切酶2抗体
规格：1尘驳/1尘濒
英文名： APEX2
别名： AP endonuclease 2; AP endonuclease XTH2; APE 2; APE2; APEX 2; APEX L2; APEX nuclease (apurinic/apyrimidinic endonuclease) 2; APEX Nuclease 2; APEX nuclease like 2; APEXL 2; APEXL2; Apurinic apyrimidin
分子量： 57kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human APEX2
交叉反应：Human, Mouse, Rat, Dog, Cow,
细胞定位：细胞核 细胞浆
嘌呤嘧啶核酸内切酶2抗体产物介绍：background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair (BER). Function: Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex嘌呤嘧啶核酸内切酶2抗体 DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. Subunit: Interacts with PCNA; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Subcellular Location: Nucleus. Cytoplasm. Mitochondrion (Probable). Note=Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. Tissue Specificity: Highly expressed in brain and kidney. 嘌呤嘧啶核酸内切酶2抗体Weakly expressed in the fetal brain. Similarity: Belongs to the DNA repair enzymes AP/ExoA family. Database links: Entrez Gene: 27301 Human Entrez Gene: 77622 Mouse Entrez Gene: 289662 Rat Omim: 300773 Human SwissProt: Q9UBZ4 Human SwissProt: Q68G58 Mouse Unigene: 659558 Human Unigene: 440275 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产物应用：嘌呤嘧啶核酸内切酶2抗体WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 染色质和核信号
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid