蛋白激酶搁/贰滨贵2础碍1抗体
规格：1尘驳/1尘濒
英文名： PKR
别名： double-stranded RNA-dependent Protein Kinase; interferon-induced, double-stranded RNA-activated protein kinase isoform a; protein kinase, interferon-inducible double stranded RNA dependent; interferon
分子量： 61kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PKR
交叉反应：Human, Mouse, Rat,
细胞定位：细胞浆
蛋白激酶搁/贰滨贵2础碍1抗体产物介绍：background: PKR is an interferon-inducible serine/threonine specific protein kinase. It is widely expressed in eukaryotic organisms and activated by double stranded RNA. Activation of PKR by dsRNAs leads to autophosphorylation at multiple sites. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 (EIF2 alpha), leading to the inhibition of protein synthesis. PKR is also involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, and also activates IKK and NFKB, thereby suppressing apoptosis. Recently, it has been reported that PKR also phosphorylates human p53 on serine 392. PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent PKR activation in association with 蛋白激酶搁/贰滨贵2础碍1抗体neuritic plaques and pyramidal neurons in the hippocampus and neocortex. Function: Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. Subunit: Homodimer. Interacts with STRBP. Interacts with DNAJC3. Inhibited by direct interaction with viral proteins such as HCV E2, HCV NS5A and influenza A NS1蛋白激酶搁/贰滨贵2础碍1抗体. Activated by the interaction with HIV-1 Tat. Forms a complex with FANCA, FANCC, FANCG and HSP70. Post-translational modifications: Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 2 DRBM (double-stranded RNA-binding) domains. Contains 1 protein kinase domain. Gene ID: 5610 Database links: Entrez Gene: 5610 Human Omim: 176871 Human SwissProt: P19525 Human SwissProt: Q52M43 Human Unigene: 131431 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 蛋白激酶R/双链RNA依赖蛋白激酶（PKR）是一种干扰素诱导的、双链RNA激活的丝氨酸/苏氨酸激酶, PKR在信号转导、细胞生长、分化和凋亡的控制中起重要作用.也有人认为：PKR是一个重要的凋亡效应物,是许多不同刺激物诱导凋亡的一个转导物。
蛋白激酶搁/贰滨贵2础碍1抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 细胞凋亡 生长因子和** 激酶和磷酸酶 细胞分化
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid