早期有丝分裂抑制蛋白1抗体
规格：1尘驳/1尘濒
英文名： Emi1
别名： Early mitotic inhibitor 1; Emi 1; F box only protein 5; F box protein 5; F box protein Fbx 5; F box protein Fbx5; F-box only protein 5; FBX5; FBX5_HUMAN; fbxo5.
分子量： 50kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human Emi1
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
细胞定位：细胞核 细胞浆
早期有丝分裂抑制蛋白1抗体产物介绍：background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding 早期有丝分裂抑制蛋白1抗体different isoforms have been identified. [provided by RefSeq, Dec 2008] Function: Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding. Subcellular Location: Nucleus. Cytoplasm. Cytoplasm > cytoskeleton >早期有丝分裂抑制蛋白1抗体 spindle. In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle. Post-translational modifications: Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression. Similarity: Contains 1 F-box domain. Contains 1 IBR-type zinc finger. Gene ID: 26271 Database links: Entrez Gene: 26271 Human GenBank: BC018905 Human Omim: 606013 Human SwissProt: Q9UKT4 Human Unigene: 520506 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
早期有丝分裂抑制蛋白1抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：信号转导 泛素
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid