缺氧诱导因子2α /HIF-2α/HIF-2-alpha抗体
规格：1尘驳/1尘濒
英文名： HIF 2 alpha
别名： Hypoxia-inducible factor -2 alpha; HIF 2 Alpha; HIF2 Alpha; HIF 2; HIF2A; AHypoxia-inducible factor 2 alpha; HIF-2 alpha; Endothelial PAS domain-containing protein 1; EPAS-1; HIF-1 alpha-like factor;
分子量： 96kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from mouse HIF 2 Al
交叉反应：Human, Mouse, Rat, Pig, Cow, Horse,
细胞定位：细胞核
缺氧诱导因子2α /HIF-2α/HIF-2-alpha抗体产物介绍：background: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Function: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) 缺氧诱导因子2α /HIF-2α/HIF-2-alpha抗体expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBPB. Subcellular Location: Nucleus (Potential). Tissue Specificity: Expressed in most tissues, with highest levels in lung, followed by heart,缺氧诱导因子2α /HIF-2α/HIF-2-alpha抗体 kidney, brain and liver. Predominantly expressed in endothelial cells. Also found in smooth muscle cells of the uterus, neurons, and brown adipose tissue. High expression in embryonic choroid plexus and kidney glomeruli. Post-translational modifications: In normoxia, is probably hydroxylated on Pro-405 and Pro-530 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. In normoxia, is hydroxylated on Asn-851 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 2 PAS (PER-ARNT-SIM) domains. Gene ID: 13819 Database links: Entrez Gene: 282711 Cow Entrez Gene: 2034 Human Entrez Gene: 13819 Mouse Entrez Gene: 100037272 Pig Entrez Gene: 29452 Rat Omim: 603349 Human SwissProt: Q99814 Human SwissProt: P97481 Mouse SwissProt: Q9JHS1 Rat Unigene: 468410 Human Unigene: 1415 Mouse Unigene: 55138 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic 缺氧诱导因子2α /HIF-2α/HIF-2-alpha抗体or diagnostic applications. HIF-2α也是机体对缺氧适应性调节中的重要调控蛋白.有学者认为：HIF-2α对肿瘤的能量代谢和新血管生成有促进作用,微血管的形成可抑制肿瘤细胞的凋亡,从而促进肿瘤的恶性发展。
产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-300 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 **学 信号转导 细胞凋亡
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid