γ-衔接蛋白相关蛋白1抗体
规格：1尘驳/1尘濒
英文名： GGA1
别名： 4930406E12Rik; ADP ribosylation factor binding protein 1; ADP ribosylation factor binding protein GGA1; ADP-ribosylation factor-binding protein GGA1; ARF binding protein 1; ARF-binding protein 1; Gamm
分子量： 70kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human GGA1
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Sheep,
细胞定位：细胞浆 细胞膜
γ-衔接蛋白相关蛋白1抗体产物介绍：background: The GGA family of proteins (Golgi-localized, ARF-binding proteins) are ubiquitous coat proteins that facilitate the trafficking of soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes by means of interactions with TGN-sorting receptors, ARF (ADP-ribosylation factor), and clathrin (1?). Members of the GGA family, GGA1,GGA2 (also known as VEAR) and GGA3, are multidomain proteins that bind mannose 6-phosphate receptors (MPRs) (1,2,4). GGAs have modular structures with an N-terminal VHS (VPS-27, Hrs, and STAM) domain followed by a GAT (GGA and TOM1) domain, a connecting hinge segment, and a C-terminal GAE (?adaptin ear) domain (5). The amino-terminal VHS domains of GGAs form complexes with the cytoplasmic domains of sorting receptors by recognizing acidic-cluster di-leucine (ACLL) sequences (3). GGA1 and GGA2 do not associate with each other, but they do colocalize on perinuclear membranes (2). The cytosolic domain of memapsin 2, but not that of memapsin 1, binds the VHS domains of GGA1 and GGA2 (6). The human GGA1 gene maps to chromosome 22 and encodes a protein that shares 45% sequence identity with GGA2 and GGA3 (1). Function: Plays a role in protein sorting and trafficking γ-衔接蛋白相关蛋白1抗体between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Subunit: Monomer. Interacts with NECAP1. Interacts with CNST (By similarity). Interacts with GGA2 and GGA3. Binds to clathrin and activated ARFs. Interacts with RABEP1 and RABGEF1. Interacts with the type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. Binds CCDC91, P200, SYNRG, EPN4, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC. Interacts with RNF11. Subcellular Location: Golgi apparatus; trans-Golgi network membrane. Endosome membrane. Tissue Specificity: Ubiquitously expressed. Post-translational modifications: Phosphorylated by γ-衔接蛋白相关蛋白1抗体CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal AC-LL motif to bind the VHS domain and to inhibit the recognition of cargo signals. Phosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated. Similarity: Belongs to the GGA protein family. Contains 1 GAE domain. Contains 1 GAT domain. Contains 1 VHS domain. Gene ID: 26088 Database links: Entrez Gene: 26088 Human Entrez Gene: 106039 Mouse Entrez Gene: 300066 Rat Omim: 606004 Human SwissProt: Q9UJY5 Human SwissProt: Q8R0H9 Mouse Unigene: 499158 Human Unigene: 251331 Mouse Unigene: 75906 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
γ-衔接蛋白相关蛋白1抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 转运蛋白
储存条件： Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid