多肽狈-乙酰氨基半乳糖转移酶12抗体
规格：1尘驳/1尘濒
英文名： GALNT12
别名： CRCS1; FLJ21212; GalNAc T12; GalNAc-T12; GalNAcT12; GALNT 12; Galnt12; GLT12_HUMAN; Polypeptide GalNAc transferase 12; Polypeptide N acetylgalactosaminyltransferase 12; Polypeptide N-acetylgalactosami
分子量： 67kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human GALNT12/
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Rabbit,
细胞定位：细胞浆 细胞膜
多肽狈-乙酰氨基半乳糖转移酶12抗体产物介绍：background: The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosamine) to serine and threonine residues onto various proteins, thereby initiating mucin-type O-linked glycosylation in the Golgi apparatus. GalNAc-T12 (Polypeptide N-acetylgalactosaminyltransferase 12), also known as UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 12, is a 581 amino acid protein that displays enzymatic activity towards non-glycosylated peptides such as Muc5Ac, Muc1a and EA2 with no detectable activity towards Muc2 and Muc7. The N-terminal domain is involved in substrate binding and manganese coordination, while the C-terminal domain is involved in UDP-Gal binding and catalytic reaction. Since GalNAc-T12 is highly expressed in stomach, pancreas, small intestine and colon, it may play a多肽狈-乙酰氨基半乳糖转移酶12抗体 significant role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs. Function: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs. Subcellular Location: Golgi apparatus membrane. Tissue Specificity: Widely expressed at different levels of expression. Highly expressed in digestive organs such as small intestine, stomach,多肽狈-乙酰氨基半乳糖转移酶12抗体 pancreas and colon. Expressed at intermediate level in testis, thyroid gland and spleen. Weakly expressed in whole brain, cerebral cortex, cerebellum, fetal brain, bone marrow, thymus, leukocytes, heart, skeletal muscle, liver, lung, esophagus, kidney, adrenal gland, mammary gland, uterus, placenta, ovary and prostate. DISEASE: Defects in GALNT12 are a cause of susceptibility to colorectal cancer type 1 (CRCS1) [MIM:608812]. Colorectal cancer is a malignancy originating either in the colon or rectum or both. Similarity: Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain. Database links: Entrez Gene: 79695 Human Entrez Gene: 230145 Mouse Omim: 610290 Human SwissProt: Q8IXK2 Human SwissProt: Q8BGT9 Mouse Unigene: 47099 Human Unigene: 132246 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
多肽狈-乙酰氨基半乳糖转移酶12抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 细胞类型标志物
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid