磷酸化真核延伸因子激酶2抗体
规格：1尘驳/1尘濒
英文名： Phospho-EEF2k (Ser359)
别名： EEF2K (phospho S359); p-EEF2K (phospho S359); Calcium/calmodulin dependent eukaryotic elongation factor 2; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase; Calmodulin dependent prot
分子量： 82kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated Synthesised phosphopeptide derived from human
交叉反应：Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit, Sheep,
细胞定位：
磷酸化真核延伸因子激酶2抗体产物介绍：background: Eukaryotic elongation factor 2 kinase (EEF2k) previously known as Ca2+/calmodulin dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor 2 (eEf2). Phosphorylation of eEF2 by eEF2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation. The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression,磷酸化真核延伸因子激酶2抗体 cellular differentiation, oogensis and malignant tumors. Function: Threonine kinase that regulates protein synthesis bycontrolling the rate of peptide chain elongation. Upon activationby a variety of upstream kinases including AMPK or TRM7,phosphorylates the elongation factor EEF2 at a single site, rendersit unable to bind ribosomes and thus inactive. In turn, the rate ofprotein synthesis is reduced. Subunit: Monomer or homodimer. Post-translational modifications: Autophosphorylated at multiple residues, Thr-348 being themajor site. Phosphorylated by 磷酸化真核延伸因子激酶2抗体AMP-activated protein kinase AMPK atSer-398 leading to EEF2K activation and protein synthesisinhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improvedprotein stability, higher EE2F phosphorylated and subsequentlyreduced rate of protein synthesis. Phosphorylation by other kinasessuch as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 atSer-366 instead decrease EEF2K activity and promote proteinsynthesis. Similarity: Belongs to the protein kinase superfamily. Alpha-type protein kinase family. Contains 1 alpha-type protein kinase domain. Database links: UniProtKB/Swiss-Prot: O00418.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化真核延伸因子激酶2抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid