胚胎外胚层发育蛋白贰贰顿抗体
规格：1尘驳/1尘濒
英文名： EED
别名： eed; EED protein; EED_HUMAN; Embryonic ectoderm development; Embryonic ectoderm development isoform a; Embryonic ECTODERM development protein homolog; hEED; OTTHUMP00000235483; OTTHUMP00000235484; OTT
分子量： 50kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human EED
交叉反应：Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep, Xenopus laevis, Xenopus tropicalis , Medaka fish
细胞定位：细胞核
胚胎外胚层发育蛋白贰贰顿抗体产物介绍：background: The transcriptional repressing Polycomb-group (PcG) and transcriptional activating trithorax-group (trxG) genes of Drosophila are part of a cellular memory system responsible for the stable inheritance of gene activity. PcG proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. EED (embryonic ectoderm development) is the human homolog of Eed, a murine PcG gene homologous to the Drosophila homeotic gene, extra sex combs. The human EED protein is 99. 5% identical to the mouse EED protein and contains seven WD repeats, which are involved in protein-protein interactions. There are two human EED transcripts that contain a putative 407-nucleotide-long intron and give rise to two HEED protein isoforms, 535 and 494 amino acids in length. EED interacts in a highly specific manner, both in vitro and in vivo, with histone deacetylase (HDAC) proteins. Function: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone 胚胎外胚层发育蛋白贰贰顿抗体H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. Subunit: Interacts with MLL (By similarity). Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts 胚胎外胚层发育蛋白贰贰顿抗体with HDAC, HDAC2, histone H1 and YY1. May interact with ITGA4, ITGAE and ITGB7. May interact with the MA protein of HIV-1. Subcellular Location: Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes. Tissue Specificity: Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer. Post-translational modifications: Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines. Similarity: Belongs to the WD repeat ESC family. Contains 7 WD repeats. Database links: Entrez Gene: 426381 Chicken Entrez Gene: 404183 Cow Entrez Gene: 8726 Human Entrez Gene: 13626 Mouse Entrez Gene: 398420 Xenopus laevis Omim: 605984 Human SwissProt: Q5ZKH3 Chicken SwissProt: Q3SZ25 Cow SwissProt: O75530 Human SwissProt: Q921E6 Mouse SwissProt: Q8UUP2 Xenopus laevis Unigene: 503510 Human Unigene: 380914 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
胚胎外胚层发育蛋白贰贰顿抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid