细胞周期后期促进蛋白亚基11抗体
规格：1尘驳/1尘濒
英文名： APC11
别名： ANAPC 11; ANAPC11; Anaphase promoting complex subunit 11; Anaphase promoting complex subunit 11 homolog (yeast); Anaphase promoting complex subunit 11 homolog; Anaphase-promoting complex sub
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human APC11
交叉反应：Human, Mouse, Rat, Dog, Pig, Sheep,
细胞定位：细胞核 细胞浆
细胞周期后期促进蛋白亚基11抗体产物介绍：background: Comprising more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to APC and activates APC’s cyclin-ubiquitination activity. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC; thus preventing APC activation. APC11 is a RING-H2 finger protein that allows for the synthesis of multiubiquitin chains in the presence of Ubiquitin carrier protein 4 (Ubc4) 细胞周期后期促进蛋白亚基11抗体and ubiquitin conjugating enzyme (E2). In addition, a heterodimeric complex of either Ubc4 or UbcH10 with APC11 and APC2 catalyzes the ubiquitination of human securin and cyclin B1. Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May recruit the E2 ubiquitin-conjugating enzymes to the complex.细胞周期后期促进蛋白亚基11抗体 Subunit: The APC/C is composed of at least 12 subunits. Interacts with the cullin domain of ANAPC2. Interacts with UBE2D2. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. Post-translational modifications: Auto-ubiquitinated. Similarity: Belongs to the RING-box family. Contains 1 RING-type zinc finger. Database links: UniProtKB/Swiss-Prot: Q9NYG5.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
细胞周期后期促进蛋白亚基11抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 细胞周期蛋白 泛素
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid