环指蛋白146抗体
规格：1尘驳/1尘濒
英文名： RNF146
别名： Dactylidin; dJ351K20.1; DKFZP434O1427; E3 ubiquitin-protein ligase rnf146; RING finger protein 146; RN146_HUMAN; RNF 146; Rnf146; RP3 351K20.1.
分子量： 39kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：笔辞濒测肠濒辞苍补濒
亚型：滨驳骋
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human RNF146 (
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
细胞定位：细胞核 细胞浆
环指蛋白146抗体产物介绍：background: The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF146 (RING finger protein 146), also known as Dactylidin, is a 359 amino acid protein that contains one RING-type zinc finger and one WWE domain. Via its RING-type zinc finger, RNF146 may play a role in transcriptional regulation and protein degradation events. Defects in the gene encoding RNF146 are associated with Alzheimer's disease (AD) and may lead to a higher risk of breast cancer. Two isoforms of RNF146 exist due to alternative splicing events. Function: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. Acts as an activator of the Wnt signaling pathway by mediating the环指蛋白146抗体 ubiquitination of poly-ADP-ribosylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate poly-ADP-ribosylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent poly-ADP-ribosylated proteins via its WWE domain and mediates their ubiquitination. Subunit: Can form homooligomers. Interacts with PARsylated AXIN1, AXIN2, BLZF1, CASC3, HIST1H1C, IPO7, LIG3, NCL, PARP1, XRCC1, XRCC5 and XRCC6. Interacts with DDB1, DHX15, IQGAP1, LRPPRC, PARP2, PRKDC, RUVBL2, TNKS1 and TNKS2. Binding often leads to interactor ubiquitination, in the presence of the appropriate E1 and E2 enzymes, and proteasomal degradation. Subcellular Location: Cytoplasm, cytosol. Nucleus. Note=Translocates to the 环指蛋白146抗体nucleus after DNA damage, such as laser-induced DNA breaks, and concentrates at DNA breaks. This translocation requires PARP1 activation and PAR-binding. Tissue Specificity: Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease. Post-translational modifications: Ubiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon poly(ADP-ribose)-binding. DISEASE: Note=Defects in RNF146 are a cause of susceptibility to breast cancer. Similarity: Contains 1 RING-type zinc finger. Contains 1 WWE domain. Database links: Entrez Gene: 81847 Human Omim: 612137 Human SwissProt: Q9NTX7 Human Unigene: 267120 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
环指蛋白146抗体产物应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：环指蛋白
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid